Puerarin protects PC12 cells against β-amyloid-induced cell injury

β-Amyloid protein (Aβ), a major protein component of brain senile plaques in Alzheimer's disease, is known to be directly responsible for the production of reactive oxygen species (ROS) and induction of apoptosis. In this study, the protective effect of puerarin, an isoflavone purified from the...

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Bibliographic Details
Published in:Cell biology international 2008-10, Vol.32 (10), p.1230-1237
Main Authors: Zhang, Hai-Ying, Liu, Yi-Heng, Wang, Hong-Quan, Xu, Jie-Hua, Hu, Hai-Tao
Format: Article
Language:English
Subjects:
Alzheimer Disease - metabolism
Alzheimer Disease - pathology
Alzheimer's disease
Amyloid beta-Peptides - pharmacology
Animals
Apoptosis
bcl-2-Associated X Protein - metabolism
Caspase 3 - metabolism
Cell injury
Humans
In Situ Nick-End Labeling
Isoflavones - pharmacology
Membrane Potential, Mitochondrial - drug effects
Neurons - drug effects
Neurons - physiology
Neuroprotective Agents - pharmacology
PC12 Cells
Peptide Fragments - pharmacology
Proto-Oncogene Proteins c-bcl-2 - metabolism
Puerarin
Rats
Reactive oxygen species
Reactive Oxygen Species - metabolism
Vasodilator Agents - pharmacology
β-Amyloid protein
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Summary:β-Amyloid protein (Aβ), a major protein component of brain senile plaques in Alzheimer's disease, is known to be directly responsible for the production of reactive oxygen species (ROS) and induction of apoptosis. In this study, the protective effect of puerarin, an isoflavone purified from the radix of the Chinese herb Pueraria lobata, on Aβ-induced rat pheochromocytoma (PC12) cultures was investigated. Although exposure of PC12 cells to 50 μM Aβ 25–35 caused significant viability loss and apoptotic rate increase, pretreatment of the cells with puerarin for 24 h reduced the viability loss and apoptotic rate. Puerarin (1 μM) significantly inhibited Aβ 25–35-induced apoptosis of PC12 cells. Preincubation of the cell with puerarin also restored the ROS and mitochondrial membrane potential levels that had been altered as a result of Aβ 25–35 treatment. Puerarin was also found to increase the Bcl-2/Bax ratio and reduce caspase-3 activation. These results suggest that puerarin could attenuate Aβ 25–35-induced PC12 cell injure and apoptosis and could also promote the survival of PC12 cells. Therefore, puerarin may act as an intracellular ROS scavenger, and its antioxidant properties may protect against Aβ 25–35-induced cell injury.
ISSN:1065-6995
1095-8355
DOI:10.1016/j.cellbi.2008.07.006