Liposomes with incorporated MHC class II/peptide complexes as antigen presenting vesicles for specific T cell activation

The purpose of this study was to design a well-characterized liposomal carrier system for multivalent antigen presentation in order to activate T cells. MHC class II molecules were loaded with peptide and subsequently reconstituted into liposomes. A FACS assay was developed to monitor peptide loadin...

Full description

Saved in:
Bibliographic Details
Published in:Pharmaceutical research 1999-02, Vol.16 (2), p.198-204
Main Authors: VAN RENSEN, A. J. M. L, WAUBEN, M. H. M, GROSFELD-STULEMEYER, M. C, VAN EDEN, W, CROMMELIN, D. J. A
Format: Article
Language:English
Subjects:
Animals
Antigen-Presenting Cells - immunology
Biological and medical sciences
Flow Cytometry
General pharmacology
Histocompatibility Antigens Class II - immunology
Histocompatibility Antigens Class II - metabolism
Humans
Hybridomas - metabolism
Interleukin-2 - biosynthesis
Liposomes
Lymphocyte Activation
Medical sciences
Mice
Peptides - immunology
Peptides - metabolism
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Rats
Rats, Inbred Lew
T-Lymphocyte Subsets - immunology
T-Lymphocyte Subsets - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The purpose of this study was to design a well-characterized liposomal carrier system for multivalent antigen presentation in order to activate T cells. MHC class II molecules were loaded with peptide and subsequently reconstituted into liposomes. A FACS assay was developed to monitor peptide loading and MHC class II incorporation in the liposomes. For in vitro testing of the resulting MHC class II/peptide liposomes, a T cell hybridoma assay was employed. The FACS assay provided a qualitative means to visualize the amount of incorporated MHC class II and peptide molecules that were oriented in the appropriate way for antigen presentation to the T cells. Interestingly, when MHC class II molecules were loaded with the appropriate peptide prior to liposome incorporation, such liposomes were fully capable of inducing IL-2 production of a T cell hybridoma. This is the first article showing that MHC class II/peptide liposomes can serve as 'artificial antigen presenting cells' for activation of a CD4+ T cell hybridoma. As compared to soluble MHC class II/peptide complexes, the multivalency of liposomal complexes may be an important advantage when studying possible applications in immunotherapy.
ISSN:0724-8741
1573-904X
DOI:10.1023/A:1018864005620