Growth of Trametes versicolor on phenol

Trametes versicolor 1 was shown to grow on phenol as its sole carbon and energy source. The culture growth and degradation ability dependence on culture medium pH value was observed. The optimal pH value of a liquid Czapek salt medium was 6.5. The investigated strain utilized completely 0.5 g/l phen...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2008-11, Vol.35 (11), p.1309-1312
Main Authors: Yemendzhiev, H, Gerginova, M, Krastanov, A, Stoilova, I, Alexieva, Z
Format: Article
Language:English
Subjects:
Biochemistry
Biodegradation
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Bioremediation
Biotechnology
Carbon
Cellular biology
Coriolus versicolor
culture media
Enzymes
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
Fungal Proteins - metabolism
Fungi
Genetic Engineering
Genetic testing
Hybridization
Inorganic Chemistry
Investigations
Labeling
Life Sciences
Lignin
microbial growth
microbial physiology
Microbiology
Microorganisms
Mixed Function Oxygenases - genetics
Mixed Function Oxygenases - metabolism
Original Paper
Phenol - metabolism
Phenols
Pollutants
Proteins
Studies
Trametes - enzymology
Trametes - genetics
Trametes - growth & development
Trametes - metabolism
Trametes versicolor
Trichosporon cutaneum
white-rot fungi
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Summary:Trametes versicolor 1 was shown to grow on phenol as its sole carbon and energy source. The culture growth and degradation ability dependence on culture medium pH value was observed. The optimal pH value of a liquid Czapek salt medium was 6.5. The investigated strain utilized completely 0.5 g/l phenol in 6 days. The dynamics of the phenol degradation process was investigated. The process was characterized by specific growth rate μmax 0.33 h⁻¹, metabolic coefficient k = 4.4, yield coefficient Y x/s = 0.23 and rate of degradation Q = 0.506 h⁻¹. The intracellular activities of phenol hydroxylase (0.333 U/mg protein) and cis,cis-muconate lactonizing enzyme (0.41 U/mg protein) were demonstrated for the first time in this fungus. In an attempt to estimate the occurrence of gene sequences in T. versicolor 1 related to phenol degradation pathway a dot blot analysis with total DNA isolated from this strain was performed. Two synthetic oligonucleotides were used as hybridizing probes. One of the probes was homologous to the 5'end of phyA gene coding for phenol hydroxylase in Trichosporon cutaneum ATCC 46490. The other probe was created on the basis of cis,cis-muconate lactonizing enzyme coding gene in T. cutaneum ATCC 58094. The results of these investigations showed that T. versicolor 1 may carry genes similar to those of Trichosporon cutaneum capable to degrade phenol.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-008-0412-z