Distribution of semaphorin IV in adult human brain

The semaphorins comprise a family of secreted and membrane bound proteins that influence development of the nervous system as well as non-neural organs. H.SemaIV was originally isolated from a homozygously deleted region involving a subset of small cell lung cancers, a neuroendocrine derived neoplas...

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Bibliographic Details
Published in:Brain research 1999-03, Vol.823 (1), p.67-79
Main Authors: Hirsch, Etienne, Hu, Ling-Jia, Prigent, Annick, Constantin, Bruno, Agid, Yves, Drabkin, Harry, Roche, Joëlle
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Alzheimer Disease - metabolism
Anatomy
Animals
Biological and medical sciences
Brain - cytology
Brain - metabolism
Cell Line
Central nervous system
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Hippocampus
Human brain
Humans
Immunohistochemistry
Insecta - cytology
Microscopy, Confocal
Middle Aged
Nerve Growth Factors - metabolism
Nerve process
Semaphorin IV
Tissue Distribution
Tumor Cells, Cultured
Vertebrates: nervous system and sense organs
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Summary:The semaphorins comprise a family of secreted and membrane bound proteins that influence development of the nervous system as well as non-neural organs. H.SemaIV was originally isolated from a homozygously deleted region involving a subset of small cell lung cancers, a neuroendocrine derived neoplasm. To investigate H.SemaIV expression, specific polyclonal antibodies directed against a unique polypeptide (amino acids 758–773) were developed and their specificity confirmed. In cell lines, H.SemaIV staining was observed in cytoplasmic granules. In the normal adult human brain, we noted three general characteristics of H.SemaIV expression. H.SemaIV was strongly present in specific nuclei or in neuronal regions arranged in defined subnuclear structures. It was also present in neurons but not glial cells or ependymocytes. Lastly, H.SemaIV was not present in cell bodies, but rather in fibers and nerve terminals. Interestingly, an altered pattern of staining was detected in brains of three patients with Alzheimer's disease.
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(99)01103-8