Differential regulation of vascular endothelial growth factor-C and its receptor in the rat hippocampus following transient forebrain ischemia

We investigated the changes in the expression of vascular endothelial growth factor-C (VEGF-C) and its receptor, VEGFR-3, in the rat hippocampus following transient forebrain ischemia. The expression profiles of VEGF-C and VEGFR-3 were very similar in the control hippocampi, where both genes were co...

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Bibliographic Details
Published in:Acta neuropathologica 2008-11, Vol.116 (5), p.517-527
Main Authors: Shin, Yoo-Jin, Choi, Jeong-Sun, Lee, Ji-Yeon, Choi, Jae-Youn, Cha, Jung-Ho, Chun, Myung-Hoon, Lee, Mun-Yong
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Astrocytes - metabolism
Astrocytes - pathology
Carotid arteries
Dentate Gyrus - blood supply
Dentate Gyrus - metabolism
Dentate Gyrus - pathology
Disease Models, Animal
Fluorescent Antibody Technique
Glial Fibrillary Acidic Protein - metabolism
Hippocampus - blood supply
Hippocampus - metabolism
Hippocampus - pathology
In Situ Hybridization
Ischemia
Ischemic Attack, Transient - physiopathology
Laboratory animals
Male
Medicine
Medicine & Public Health
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Neurogenesis
Neuroglia - metabolism
Neuroglia - pathology
Neurons - metabolism
Neurons - pathology
Neurosciences
Original Paper
Pathology
Prosencephalon - blood supply
Pyramidal Cells - metabolism
Pyramidal Cells - pathology
Rats
Rats, Sprague-Dawley
Receptors, Vascular Endothelial Growth Factor - genetics
Receptors, Vascular Endothelial Growth Factor - metabolism
Reperfusion - methods
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Up-Regulation
Vascular endothelial growth factor
Vascular Endothelial Growth Factor C - genetics
Vascular Endothelial Growth Factor C - metabolism
Vascular Endothelial Growth Factor Receptor-3 - genetics
Vascular Endothelial Growth Factor Receptor-3 - metabolism
Veins & arteries
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Summary:We investigated the changes in the expression of vascular endothelial growth factor-C (VEGF-C) and its receptor, VEGFR-3, in the rat hippocampus following transient forebrain ischemia. The expression profiles of VEGF-C and VEGFR-3 were very similar in the control hippocampi, where both genes were constitutively expressed in neurons in the pyramidal cell and granule cell layers. The spatiotemporal expression pattern of VEGF-C was similar to that of VEGFR-3 in the ischemic hippocampus, and in the CA1 and dentate hilar regions both VEGF-C and VEGFR-3 were strongly expressed in activated glial cells rather than in neurons. Most of the activated glial cells expressing both genes were reactive astrocytes, although some were a subpopulation of brain macrophages. In the dentate gyrus, however, VEGFR-3 expression was transiently increased in the innermost layer of granule cells on days 7–10 after reperfusion, coinciding with an increase in polysialylated neural cell adhesion molecule staining—a marker for immature neurons. These data suggest that VEGF-C may be involved in glial reaction via paracrine or autocrine mechanisms in the ischemic brain and may carry out specific roles in adult hippocampal neurogenesis during ischemic insults.
ISSN:0001-6322
1432-0533
DOI:10.1007/s00401-008-0423-x