Inflammatory Cytokines Provide a Third Signal for Activation of Naive CD4+ and CD8+ T Cells

The effects of inflammatory cytokines on naive T cells have been studied using MHC protein/peptide complexes on microspheres, thus avoiding the use of APCs whose functions may be affected by the cytokines. IL-1, but not IL-12, increased proliferation of CD4+ T cells in response to Ag and IL-2, which...

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Bibliographic Details
Published in:The Journal of immunology (1950) 1999-03, Vol.162 (6), p.3256-3262
Main Authors: Curtsinger, Julie M, Schmidt, Clint S, Mondino, Anna, Lins, Debra C, Kedl, Ross M, Jenkins, Marc K, Mescher, Matthew F
Format: Article
Language:English
Subjects:
AIDS/HIV
Animals
Antigens - physiology
B7-1 Antigen - physiology
CD4-Positive T-Lymphocytes - cytology
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - cytology
CD8-Positive T-Lymphocytes - immunology
Cell Differentiation - immunology
Clone Cells - immunology
Cytokines - physiology
Cytotoxicity, Immunologic
Egg Proteins - immunology
Inflammation - immunology
Interleukin-1 - physiology
Interleukin-12 - physiology
Interleukin-2 - physiology
Interphase - immunology
Lymphocyte Activation
Mice
Ovalbumin - immunology
Peptide Fragments
Signal Transduction - immunology
T-Lymphocytes, Regulatory - cytology
T-Lymphocytes, Regulatory - immunology
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Summary:The effects of inflammatory cytokines on naive T cells have been studied using MHC protein/peptide complexes on microspheres, thus avoiding the use of APCs whose functions may be affected by the cytokines. IL-1, but not IL-12, increased proliferation of CD4+ T cells in response to Ag and IL-2, which is consistent with effects on in vivo priming of CD4+ cells. In contrast, proliferation of CD8+ T cells to Ag and IL-2 required IL-12, and IL-12 replaced adjuvant in stimulating an in vivo response to peptide. These results support a model in which distinct inflammatory cytokines act directly on naive CD4+ and CD8+ T cells to provide a third signal, along with Ag and IL-2, to optimally activate differentiation and clonal expansion.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.162.6.3256