Loss of heterozygosity of APC and DCC tumor suppressor genes in human sporadic colon cancer

We examined 36 cases of human sporadic colon carcinoma and corresponding normal tissue samples to evaluate loss of heterozygosity at the APC and DCC tumor suppressor genes loci using restriction fragment length polymorphism polymerase chain reaction and variable nucleotide tandem repeat analysis. Ob...

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Bibliographic Details
Published in:Journal of molecular medicine (Berlin, Germany) Germany), 1999-03, Vol.77 (3), p.316-321
Main Authors: STURLAN, S, KAPITANOVIC, S, KOVACEVIC, D, LUKAC, J, SPAVENTI, S, SPAVENTI, R, PAVELIC, K
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenomatous Polyposis Coli Protein
Biological and medical sciences
Cell Adhesion Molecules - genetics
Colon cancer
Colonic Neoplasms - genetics
Cytoskeletal Proteins - genetics
DCC Receptor
DNA Primers
Gastroenterology. Liver. Pancreas. Abdomen
Genes, Tumor Suppressor
Humans
Loss of Heterozygosity
Medical sciences
Microsatellite Repeats
Polymorphism, Restriction Fragment Length
Receptors, Cell Surface
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumor Suppressor Proteins
Tumors
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Summary:We examined 36 cases of human sporadic colon carcinoma and corresponding normal tissue samples to evaluate loss of heterozygosity at the APC and DCC tumor suppressor genes loci using restriction fragment length polymorphism polymerase chain reaction and variable nucleotide tandem repeat analysis. Observed informativity was 83% for APC and 75% for DCC. DNA from 6 (20%) of 30 informative tumors exhibited loss of heterozygosity at the APC locus. Loss of heterozygosity at the DCC locus was observed in 7 (26%) of 27 informative tumor DNAs. Our results support the view that malignant progression is a consequence of more than one genetic change and suggest that inactivation of APC and DCC genes plays a role in a multistep process of colon tumor progression.
ISSN:0946-2716
1432-1440
DOI:10.1007/s001090050357