In Vivo UVA-1 and UVB Irradiation Differentially Perturbs the Antigen-Presenting Function of Human Epidermal Langerhans Cells

Ultraviolet B (UVB, 290–320 nm) radiation is known to suppress the immune function of epidermal Langerhans cells. We have recently described that in vitro UVB irradiation perturbs the antigen-presenting cell function of Langerhans cells by inhibiting their expression of functional B7 costimulatory m...

Full description

Saved in:
Bibliographic Details
Published in:Journal of investigative dermatology 1999-03, Vol.112 (3), p.322-325
Main Authors: Dittmar, Henning C., Weiss, Johannes M., Termeer, Christian C., Denfeld, Ralf W., Schöpf, Erwin, Simon, Jan C., Wanner, Marcus B., Skov, Lone, Barker, Jonathan Nwn, Baadsgaard, Ole
Format: Article
Language:English
Subjects:
Antigen Presentation - radiation effects
Antigen-Presenting Cells - metabolism
Antigen-Presenting Cells - physiology
Antigen-Presenting Cells - radiation effects
Antigens, CD - metabolism
B7-2 Antigen
Biological and medical sciences
Biological effects of radiation
CD28
CD80
CD86
Epidermis - radiation effects
Fundamental and applied biological sciences. Psychology
Humans
Langerhans Cells - metabolism
Langerhans Cells - physiology
Langerhans Cells - radiation effects
Membrane Glycoproteins - metabolism
Non ionizing radiations. Hertzian waves. Biooptics
Skin - cytology
Skin - metabolism
Skin - radiation effects
tetanus toxoid
Tissues, organs and organisms biophysics
Ultraviolet Rays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ultraviolet B (UVB, 290–320 nm) radiation is known to suppress the immune function of epidermal Langerhans cells. We have recently described that in vitro UVB irradiation perturbs the antigen-presenting cell function of Langerhans cells by inhibiting their expression of functional B7 costimulatory molecules (B7–1, B7–2). The aim of this study was to determine wavelength-specific UV effects on Langerhans cells function in vivo, specifically UVB and UVA-1. To address this issue, volunteers were irradiated on the sunprotected volar aspects of their forearms with 3 × minimal erythema dose of UVB (Philips TL-12) and UVA-1 (UVASUN 5000 Mutzhaas). Langerhans cells were isolated from suction blister roofs immediately following irradiation. Langerhans cells isolated from UVB- but not from UVA-1-irradiated skin failed to activate naïve resting allogeneic T cells (mixed epidermal cell leukocyte reaction) or primed tetanus toxoid reactive autologous T cells. Langerhans cells isolated from sham-irradiated or UVA-1-irradiated skin strongly upregulated B7–2 molecules during short-term tissue culture. By contrast, Langerhans cells from UVB-irradiated skin did not upregulate B7–2 molecules. Furthermore, exogenous stimulation of the B7 pathway by anti-CD28 stimulatory monoclonal antibodies restored the capacity of UVB-irradiated Langerhans cells to activate both alloreactive and tetanus toxoid-reactive T cells, implying suppressed antigen-presenting cell activities and perturbed B7 expression of Langerhans cells isolated from UVB-irradiated skin are related. Those studies demonstrate that in vivo UVB, but not UVA-1, interferes with the activation-dependent upregulation of B7 molecules on Langerhans cells, which in turn is of functional relevance for the perturbed antigen-presenting cell function of Langerhans cells within UVB- but not UVA-1-irradiated skin.
ISSN:0022-202X
1523-1747
DOI:10.1046/j.1523-1747.1999.00527.x