Identification of the repeated number of C and D regions of tyrosine phosphorylation motifs in Helicobacter pylori cagA using multiplex PCR

ABSTRACT Various tyrosine phosphorylation motif regions of H. pylori cagA exist. The number of these regions was found to have some influence on cell signaling, which was found to be more pronounced when in D (ESS) region than in C (WSS) region. A molecular biological method with multiplex PCR was d...

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Bibliographic Details
Published in:Microbiology and immunology 2008-10, Vol.52 (10), p.479-483
Main Authors: An, Byungrak, Lee, Gyusang, Lim, Kwanhun, Moon, Byung Soo, Kim, Jong Bae
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Amino Acid Sequence
Antigens, Bacterial - genetics
Antigens, Bacterial - metabolism
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Base Sequence
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
ESS
Female
Helicobacter Infections - immunology
Helicobacter pylori - genetics
Helicobacter pylori - metabolism
Helicobacter pylori - pathogenicity
Helicobacter pylori cagA
Humans
Male
Molecular Sequence Data
multiplex PCR
Phosphorylation
Polymerase Chain Reaction
Sequence Alignment
Stomach Diseases - microbiology
WSS
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Summary:ABSTRACT Various tyrosine phosphorylation motif regions of H. pylori cagA exist. The number of these regions was found to have some influence on cell signaling, which was found to be more pronounced when in D (ESS) region than in C (WSS) region. A molecular biological method with multiplex PCR was developed to distinguish C and D regions, and to identify the repetition number of tyrosine phosphorylation of the cagA gene. Multiplex PCR using novel primer sets was performed on 73 strains of H. pylori isolated from Korean patients with upper gastrointestinal diseases. The Western cagA was identified in only 3 strains (4.1%) whereas East Asia cagA was identified in 69 strains (94.5%). These results were reconfirmed through a sequencing analysis. The method developed in this study would be useful for monitoring the repeated number of C and D regions of tyrosine phosphorylation motifs in H. pylori cagA.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.2008.00064.x