Cyclin A1 Expression in Leukemia and Normal Hematopoietic Cells

Human cyclin A1 is a newly cloned, tissue-specific cyclin that is prominently expressed in normal testis. In this study, we showed that cyclin A1 was highly expressed in a subset of leukemia samples from patients. The highest frequency of cyclin A1 overexpression was observed in acute myelocytic leu...

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Bibliographic Details
Published in:Blood 1999-03, Vol.93 (6), p.2067-2074
Main Authors: Yang, Rong, Nakamaki, Tsuyoshi, Lübbert, Michael, Said, Jonathan, Sakashita, Akiko, Freyaldenhoven, Bettina S., Spira, Susan, Huynh, Vong, Müller, Carsten, Koeffler, H. Phillip
Format: Article
Language:English
Subjects:
Antigens, CD34 - analysis
Biological and medical sciences
Blotting, Northern
Cell Differentiation
Cyclin A - analysis
Cyclin A - genetics
Cyclin A1
Flow Cytometry
Fluorescent Antibody Technique
Gene Expression
Hematologic and hematopoietic diseases
Hematopoietic Stem Cells - immunology
Hematopoietic Stem Cells - metabolism
Humans
Leukemia - metabolism
Leukemia, Myeloid, Acute - metabolism
Leukemia, Promyelocytic, Acute - metabolism
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Medical sciences
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
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Summary:Human cyclin A1 is a newly cloned, tissue-specific cyclin that is prominently expressed in normal testis. In this study, we showed that cyclin A1 was highly expressed in a subset of leukemia samples from patients. The highest frequency of cyclin A1 overexpression was observed in acute myelocytic leukemias, especially those that were at the promyelocyte (M3) and myeloblast (M2) stages of development. Cyclin A1 expression was also detected in normal CD34+progenitor cells. The expression of cyclin A1 increased when these cells were stimulated to undergo myeloid differentiation in vitro. Taken together, our observations suggest that cyclin A1 may have a role in hematopoiesis. High levels of cyclin A1 expression are especially associated with certain leukemias blocked at the myeloblast and promyelocyte stages of differentiation.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V93.6.2067.406k26_2067_2074