Alterations in insulin-like growth factor-1 gene and protein expression and type 1 insulin-like growth factor receptors in the brains of ageing rats

Ageing in mammals is characterized by a decline in plasma levels of insulin-like growth factor-1 that appears to contribute to both structural and functional changes in a number of tissues. Although insulin-like growth factor-1 has been shown to provide trophic support for neurons and administration...

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Bibliographic Details
Published in:Neuroscience 1999, Vol.88 (1), p.269-279
Main Authors: Sonntag, W.E, Lynch, C.D, Bennett, S.A, Khan, A.S, Thornton, P.L, Cooney, P.T, Ingram, R.L, McShane, T, Brunso-Bechtold, J.K
Format: Article
Language:English
Subjects:
ageing
Aging - metabolism
Animals
Arterioles - growth & development
Arterioles - metabolism
Brain - blood supply
Brain - growth & development
Brain - metabolism
Cerebral Cortex - blood supply
Cerebrovascular Circulation
cerebrovasculature
Gene Expression Regulation
Hippocampus - metabolism
in situ hybridization
Insulin-Like Growth Factor I - genetics
Insulin-Like Growth Factor I - metabolism
insulin-like growth factor-1
Male
messenger RNA
Microcirculation - growth & development
Microcirculation - metabolism
Protein Biosynthesis
Rats
Rats, Inbred BN
Rats, Inbred F344
Receptor, IGF Type 1 - genetics
Receptor, IGF Type 1 - metabolism
RNA, Messenger - analysis
RNA, Messenger - genetics
Transcription, Genetic
type 1 insulin-like growth factor receptors
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Summary:Ageing in mammals is characterized by a decline in plasma levels of insulin-like growth factor-1 that appears to contribute to both structural and functional changes in a number of tissues. Although insulin-like growth factor-1 has been shown to provide trophic support for neurons and administration of insulin-like growth factor-1 to ageing animals reverses some aspects of brain ageing, age-related changes in insulin-like growth factor-1 or type 1 insulin-like growth factor receptors in brain have not been well documented. In this series of studies, insulin-like growth factor-1 messenger RNA and protein concentrations, and type 1 insulin-like growth factor receptor levels were analysed in young (three to four- and 10–12-month-old), middle-aged (19–20-month-old) and old (29–32-month-old) Fisher 344× Brown Norway rats. Localization of insulin-like growth factor-1 messenger RNA throughout the lifespan revealed that expression was greatest in arteries, arterioles, and arteriolar anastomoses with greater than 80% of these vessels producing insulin-like growth factor-1 messenger RNA. High levels of expression were also noted in the meninges. No age-related changes were detected by either in situ hybridization or quantitative dot blot analysis of cortical tissue. However, analysis of insulin-like growth factor-1 protein levels in cortex analysed after saline perfusion indicated a 36.5% decrease between 11 and 32 months-of-age ( P
ISSN:0306-4522
1873-7544
DOI:10.1016/S0306-4522(98)00192-4