An IgG avidity ELISA to discriminate between recent and chronic Neospora caninum infection

Department of Ruminant Medicine and Veterinary Epidemiology, Swedish University of Agricultural Sciences, Uppsala. The avidity of IgG antibodies directed to Neospora caninum was measured using an IgG avidity enzyme-linked immunosorbent assay (ELISA) employing N. caninum proteins incorporated into im...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 1999-01, Vol.11 (1), p.41-44
Main Authors: Bjorkman, C, Naslund, K, Stenlund, S, Maley, SW, Buxton, D, Uggla, A
Format: Article
Language:English
Subjects:
Animals
Antibodies, Protozoan - blood
Antibody Affinity
Antigens, Protozoan - immunology
Cattle
Cattle Diseases - diagnosis
Cattle Diseases - immunology
Cattle Diseases - physiopathology
Chronic Disease
Coccidiosis - diagnosis
Coccidiosis - immunology
Coccidiosis - veterinary
Diagnosis, Differential
Enzyme-Linked Immunosorbent Assay
Immunoglobulin G - blood
Neospora - immunology
Time Factors
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Summary:Department of Ruminant Medicine and Veterinary Epidemiology, Swedish University of Agricultural Sciences, Uppsala. The avidity of IgG antibodies directed to Neospora caninum was measured using an IgG avidity enzyme-linked immunosorbent assay (ELISA) employing N. caninum proteins incorporated into immunostimulating complexes as antigen. In this ELISA, low-affinity antibodies were eluted by adding an incubation step with urea after the serum incubation. The antibody titers obtained with and without incubation with urea were then used to calculate the IgG avidity values. Analysis of sequential sera collected from experimentally infected calves revealed that the avidity increased during the course of infection. Three weeks after infection, the IgG avidity was 9-18%, and 24 weeks later it had increased to 58-76%. Cattle naturally infected for more than 6 months all had avidities >50%. The results in this study, however preliminary, indicate that the IgG avidity ELISA can be used to discriminate between recent and chronic N. caninum infections and may therefore be a valuable complement to IgG assays in epidemiologic studies of N. caninum infection in cattle.
ISSN:1040-6387
1943-4936
DOI:10.1177/104063879901100106