Novel Polymorphism of the 5-Lipoxygenase Activating Protein (FLAP) Promoter Gene Associated with Asthma

The human 5-lipoxygenase activating protein (FLAP) gene is one of the key genes involved in the production of the cysteinyl-leukotrienes. We studied novel polymorphism of the FLAP promoter gene and attempted to clarify the relationship between this polymorphism and asthma. We sequenced the FLAP prom...

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Bibliographic Details
Published in:Molecular cell biology research communications 1999-07, Vol.2 (1), p.32-35
Main Authors: Koshino, Takeshi, Takano, Saeko, Kitani, Seiichi, Ohshima, Nobuharu, Sano, Yasuyuki, Takaishi, Toshiaki, Hirai, Koichi, Yamamoto, Kazuhiko, Morita, Yutaka
Format: Article
Language:English
Subjects:
5-Lipoxygenase-Activating Proteins
Arachidonate 5-Lipoxygenase - genetics
Asthma - genetics
Carrier Proteins - genetics
Humans
Membrane Proteins - genetics
Polymerase Chain Reaction
Polymorphism, Genetic
Promoter Regions, Genetic
Sequence Analysis, DNA
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Summary:The human 5-lipoxygenase activating protein (FLAP) gene is one of the key genes involved in the production of the cysteinyl-leukotrienes. We studied novel polymorphism of the FLAP promoter gene and attempted to clarify the relationship between this polymorphism and asthma. We sequenced the FLAP promoter region, containing the −170 to +46-bp sequence from the translational start codon, and found two homozygotes of novel alleles in the polyadenyl region which showed 21 A repeats and 18 A repeats, respectively. The frequency of the 21 A repeats was 52/71 (73.2%) in asthmatics and 39/71 (54.9%) in control subjects. The difference between these frequencies was statistically significant (P = 0.035). This is the first report of FLAP promoter gene polymorphism associated with asthma. Our data suggest that FLAP promoter gene polymorphism might play a crucial role in the pathogenesis of asthma.
ISSN:1522-4724
1522-4732
DOI:10.1006/mcbr.1999.0147