ELISA underestimates measles antibody seroprevalence in US military recruits

Abstract The prevalence of antibodies to measles, mumps, and rubella in US military recruits is of importance to public health leaders. We performed ELISA testing using a commercially available product on samples from 537 recruits obtained in 1998, of which 437 were positive (81%). We then performed...

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Bibliographic Details
Published in:Vaccine 2008-09, Vol.26 (38), p.4877-4878
Main Authors: Mancuso, James D, Krauss, Margot R, Audet, Susette, Beeler, Judy A
Format: Article
Language:English
Subjects:
Allergy and Immunology
Antibodies, Viral - blood
Enzyme-Linked Immunosorbent Assay - standards
Estimates
Humans
Immunizations
Measles
Measles - diagnosis
Measles - epidemiology
Measles - immunology
Measles virus - immunology
Military Personnel
Mumps
Seroepidemiologic Studies
Seroepidemiology
United States - epidemiology
Vaccination
Validation studies
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Summary:Abstract The prevalence of antibodies to measles, mumps, and rubella in US military recruits is of importance to public health leaders. We performed ELISA testing using a commercially available product on samples from 537 recruits obtained in 1998, of which 437 were positive (81%). We then performed a validation study in a subsample of the population using plaque reduction neutralization (PRN) to assess misclassification error. This resulted in a corrected estimate of the prevalence of immunity to measles of 96% (95% CI: 92–100%). The military vaccinates a percentage of recruits who are likely to be immune if more sensitive testing, such as PRN, was used.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2008.06.028