Cytokine adsorption/release on uniform magnetic nanoparticles for localized drug delivery

Attachment of cytokines to magnetic nanoparticles has been developed as a system for controlled local drug release in cancer therapy. We studied the adsorption/release of murine interferon gamma (IFN-γ) on negatively charged magnetic nanoparticles prepared by three different methods, including copre...

Full description

Saved in:
Bibliographic Details
Published in:Journal of controlled release 2008-09, Vol.130 (2), p.168-174
Main Authors: Mejías, Raquel, Costo, Rocío, Roca, Alejandro G., Arias, Cristina F., Veintemillas-Verdaguer, Sabino, González-Carreño, Teresita, del Puerto Morales, María, Serna, Carlos J., Mañes, Santos, Barber, Domingo F.
Format: Article
Language:English
Subjects:
Adsorption
Animals
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacology
Antineoplastic Agents - therapeutic use
Biological and medical sciences
Cytokines
Drug Carriers - chemistry
Drug Compounding
Drug delivery
Drug Delivery Systems - methods
Drug Stability
General pharmacology
Hydrogen-Ion Concentration
Immunomodulation
Interferon-gamma - chemistry
Interferon-gamma - pharmacology
Interferon-gamma - therapeutic use
Iron oxide
Macrophages, Peritoneal - drug effects
Macrophages, Peritoneal - immunology
Magnetics
Medical sciences
Mice
Mice, Inbred C57BL
Microscopy, Electron, Transmission
Nanoparticle
Nanoparticles - chemistry
Neoplasms - drug therapy
Neoplasms - immunology
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Surface modifications
Surface Properties
X-Ray Diffraction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Attachment of cytokines to magnetic nanoparticles has been developed as a system for controlled local drug release in cancer therapy. We studied the adsorption/release of murine interferon gamma (IFN-γ) on negatively charged magnetic nanoparticles prepared by three different methods, including coprecipitation, decomposition in organic media, and laser pyrolysis. To facilitate IFN-γ adsorption, magnetic nanoparticles were surface modified by distinct molecules to achieve high negative charge at pH 7, maintaining small aggregate size and stability in biological media. We analyzed carboxylate-based coatings and studied the colloidal properties of the resulting dispersions. Finally, we incubated the magnetic dispersions with IFN-γ and determined optimal conditions for protein adsorption onto the particles, as well as the release capacity at different pH and as a function of time. Particles prepared by decomposition in organic media and further modified with dimercaptosuccinic acid showed the most efficient adsorption/release capacity. IFN-γ adsorbed on these nanoparticles would allow concentration of this protein or other biomolecules at specific sites for treatment of cancer or other diseases.
ISSN:0168-3659
1873-4995
DOI:10.1016/j.jconrel.2008.05.028