A novel preparation to study rat pancreatic spinal and vagal mechanosensitive afferents in vitro

The management of pancreatic pain is a significant clinical problem so understanding of how sensory signals are generated in pancreatic tissue is fundamental. We aimed to characterize mechanosensitive and chemosensitive properties of pancreatic spinal and vagal afferents in vitro. Spinal and vagal a...

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Bibliographic Details
Published in:Neurogastroenterology and motility 2008-09, Vol.20 (9), p.1060-1069
Main Authors: Schloithe, A. C., Sutherland, K., Woods, C. M., Blackshaw, L. A., Davison, J. S., Toouli, J., Saccone, G. T. P.
Format: Article
Language:English
Subjects:
Action Potentials - physiology
afferents
Animals
chemosensitive
Electrophysiology
Gastrointestinal Tract - innervation
Mechanoreceptors - cytology
Mechanoreceptors - physiology
mechanosensitive
Neurons, Afferent - cytology
Neurons, Afferent - physiology
Pancreas - innervation
rat
Rats
Rats, Sprague-Dawley
Spinal Cord - cytology
splanchnic
Stress, Mechanical
vagus
Vagus Nerve - cytology
Vagus Nerve - physiology
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Summary:The management of pancreatic pain is a significant clinical problem so understanding of how sensory signals are generated in pancreatic tissue is fundamental. We aimed to characterize mechanosensitive and chemosensitive properties of pancreatic spinal and vagal afferents in vitro. Spinal and vagal afferent preparations from Sprague–Dawley rats were established incorporating the left splanchnic nerve or vagus nerves respectively. The common bile duct was cannulated for distension of the pancreatic duct with fluid. Nerve discharge evoked by blunt probing, duct distension or electrical stimulation was obtained from teased nerve bundles using standard extra‐cellular recording. Discharge from 197 spinal afferent bundles was recorded, of which 57% displayed spontaneous activity. Blunt probing revealed 61 mechanosensitive receptive fields which were associated primarily with arteries/blood vessels (33/61) and the parenchyma (22/61). All mechanosensitive responses were slowly adapting, with 33% continuing to discharge after termination of the stimulus and 60% displaying a response threshold
ISSN:1350-1925
1365-2982
DOI:10.1111/j.1365-2982.2008.01141.x