CRH mRNA expression in the hypothalamic paraventricular nucleus is inhibited despite the activation of the hypothalamo-pituitary-adrenal axis during starvation

Abstract Corticotropin-releasing hormone (CRH) is one of the anorexigenic neuropeptides, and indeed the expression of hypothalamic CRH is known to be inhibited by starvation. To clarify whether elevated plasma glucocorticoid during starvation is responsible for the CRH suppression, we examined the e...

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Bibliographic Details
Published in:Brain research 2008-09, Vol.1228, p.107-112
Main Authors: Nishiyama, Mitsuru, Makino, Shinya, Iwasaki, Yasumasa, Tanaka, Yasushi, Nazarloo, Hossein Pournajafi, Kaneda, Tadafumi, Asaba, Koichi, Hashimoto, Kozo
Format: Article
Language:English
Subjects:
Adrenalectomy - methods
Adrenocorticotropic Hormone - blood
Animals
Biological and medical sciences
Corticosterone
Corticosterone - administration & dosage
Corticosterone - blood
Corticosterone - pharmacology
Corticosterone-releasing hormone
Corticotropin-Releasing Hormone - genetics
Corticotropin-Releasing Hormone - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression - drug effects
Glucose - analysis
Hormones and neuropeptides. Regulation
Hypothalamo-Hypophyseal System - drug effects
Hypothalamo-Hypophyseal System - metabolism
Hypothalamo-Hypophyseal System - physiology
Hypothalamus - cytology
Hypothalamus - metabolism
Hypothalamus. Hypophysis. Epiphysis. Urophysis
Immunoradiometric Assay - methods
In Situ Hybridization
Injections, Subcutaneous
Insulin - blood
Male
Neurology
Paraventricular Hypothalamic Nucleus - cytology
Paraventricular Hypothalamic Nucleus - drug effects
Paraventricular Hypothalamic Nucleus - metabolism
Paraventricular nucleus
Pituitary-Adrenal System - drug effects
Pituitary-Adrenal System - metabolism
Pituitary-Adrenal System - physiology
Rats
Rats, Wistar
RNA, Messenger - genetics
RNA, Messenger - metabolism
Starvation
Starvation - physiopathology
Vertebrates: endocrinology
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Summary:Abstract Corticotropin-releasing hormone (CRH) is one of the anorexigenic neuropeptides, and indeed the expression of hypothalamic CRH is known to be inhibited by starvation. To clarify whether elevated plasma glucocorticoid during starvation is responsible for the CRH suppression, we examined the expression level of hypothalamic CRH mRNA after food deprivation in adrenalectomized, plasma corticosterone (B)-clamped animals. Male Wistar rats were divided into 2 groups: one group had adrenalectomy (ADX) and B pellet implantation (ADX + B, n = 42), and the other group had only sham operation (sham, n = 42). Rats were then treated with either ad libitum food supply or food deprivation for up to 96 h. The expression of CRH mRNA in the paraventricular nucleus (PVN) was estimated by in situ hybridization. After food deprivation, mean plasma B level was markedly elevated in sham group, but almost clamped in the ADX + B group. In this experimental condition, CRH mRNA in the PVN was significantly decreased in the sham group, whereas no change was obtained in the ADX + B group. Our data suggest the decrease in CRH mRNA seems to be related to the elevated glucocorticoid level during starvation. The status of hyperadrenocorticism without activation of CRH led us to speculate that adrenocortical function is predominant in the hypothalamic-pituitary-adrenal (HPA) axis during starvation.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2008.06.065