Human herpesvirus-6 infection induces the reorganization of membrane microdomains in target cells, which are required for virus entry

Abstract Cell-membrane raft microdomains are important for successful infection by several viruses. However, their role in the cell-entry process of human herpesvirus-6 (HHV-6) is unknown. Here we tested whether HHV-6 requires cell-membrane rafts for its entry. When cell-membrane rafts were disrupte...

Full description

Saved in:
Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2008-09, Vol.378 (2), p.265-271
Main Authors: Tang, Huamin, Kawabata, Akiko, Takemoto, Masaya, Yamanishi, Koichi, Mori, Yasuko
Format: Article
Language:English
Subjects:
CD46
Cell Line
Cell Membrane - chemistry
Cholesterol - metabolism
Entry
gQ1
Herpesvirus 6, Human - physiology
HHV-6
Human herpesvirus 6
Humans
Infectious Disease
Lipid rafts
Membrane Cofactor Protein - analysis
Membrane Microdomains - metabolism
T-Lymphocytes - chemistry
T-Lymphocytes - virology
Viral Structural Proteins - analysis
Virus Attachment
Virus Internalization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Cell-membrane raft microdomains are important for successful infection by several viruses. However, their role in the cell-entry process of human herpesvirus-6 (HHV-6) is unknown. Here we tested whether HHV-6 requires cell-membrane rafts for its entry. When cell-membrane rafts were disrupted by cholesterol depletion, target-cell entry by HHV-6 was inhibited, although the virus bound normally to the cells. HHV-6 infectivity was partially rescued by adding exogenous cholesterol. Interestingly, the HHV-6 cellular receptor, CD46, was found in the rafts after virus attachment, but not in the rafts of uninfected cells, indicating that HHV-6 infection induces the re-location of its receptor into the rafts. Furthermore, glycoprotein Q1, part of a viral glycoprotein complex that binds CD46, was also associated with rafts immediately after infection. These data suggest that cellular-membrane lipid rafts are important in viral entry and that HHV-6 may enter the target cells via the rafts.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2008.05.028