Stress alters the cellular and proteomic compartments of bovine bronchoalveolar lavage fluid

The stresses of transportation, weaning and commingling are associated with an increased incidence of bacterial and viral pneumonia in cattle. Proteins expressed in the epithelial lining fluid (ELF) of the lungs, in conjunction with resident leukocytes, represent the first line of defence against op...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 2008-09, Vol.125 (1), p.111-125
Main Authors: Mitchell, Gordon B., Clark, Mary Ellen, Siwicky, Megan, Caswell, Jeff L.
Format: Article
Language:English
Subjects:
acute phase proteins
Acute phase response
animal stress
animal transport
animal well-being
Animals
body fluids
bronchi
Bronchoalveolar lavage
Bronchoalveolar Lavage Fluid - chemistry
Bronchoalveolar Lavage Fluid - cytology
calves
Carrier Proteins - biosynthesis
Carrier Proteins - genetics
Cattle
Cattle - blood
Cattle - metabolism
Electrophoresis, Gel, Two-Dimensional - veterinary
Female
haptoglobins
Haptoglobins - metabolism
Hemeproteins - biosynthesis
Hemeproteins - genetics
inflammation
leukocytes
Lipopolysaccharides - pharmacology
Male
neutrophils
Neutrophils - metabolism
odor compounds
Oxidative stress
protein synthesis
Proteomics
Random Allocation
Receptors, Odorant - biosynthesis
Receptors, Odorant - genetics
Respiratory Mucosa - metabolism
Respiratory Mucosa - pathology
respiratory tract diseases
Reverse Transcriptase Polymerase Chain Reaction - veterinary
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Stress
Stress, Physiological - blood
Stress, Physiological - metabolism
Stress, Physiological - pathology
Stress, Physiological - veterinary
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Summary:The stresses of transportation, weaning and commingling are associated with an increased incidence of bacterial and viral pneumonia in cattle. Proteins expressed in the epithelial lining fluid (ELF) of the lungs, in conjunction with resident leukocytes, represent the first line of defence against opportunistic pathogens, and stress-induced alterations in their expression may reveal markers of disease susceptibility. Bronchoalveolar lavage fluid was sampled in weaned and transported calves and ELF protein expression was compared to a control group of calves using two-dimensional electrophoresis (2DE). Serum and pulmonary haptoglobin were increased following stress concurrent with the number of blood neutrophils. Using 2DE, significant changes in expression were observed in spots identified by mass spectrometry as annexin A1 and A5, odorant-binding protein (OBP), isocitrate dehydrogenase, fibrinogen, heme-binding protein, α-2-HS-glycoprotein, α-1-antichymotrypsin and albumin. Quantification of OBP mRNA by real-time RT-PCR and OBP protein by western blot revealed gender-dependent differences in relative OBP expression in response to stress. These findings reveal stress-associated protein changes in pulmonary ELF and suggest a mechanism through which stress alters respiratory disease susceptibility.
ISSN:0165-2427
1873-2534
DOI:10.1016/j.vetimm.2008.05.005