Detection of antibodies against hepatitis A virus in eluates of blood spotted on filter-paper: a pilot study in Rio de Janeiro, Brazil

The validity of blood spotted on to filter-paper (BSOFP) eluates for the detection of antibodies against hepatitis A virus (HAV) was investigated in 718 individuals (children and adults) during a field study in a small area in Rio de Janeiro State, Brazil. Serum samples were considered the ‘gold sta...

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Bibliographic Details
Published in:Transactions of the Royal Society of Tropical Medicine and Hygiene 1999-07, Vol.93 (4), p.401-404
Main Authors: de Almeida, Liz Maria, Azevedo, Raymundo Soares, Marandino Guimarães, Angélica Arpon, da Silva Freire Coutinho, Evandro, Struchiner, Claudio José, Massad, Eduardo
Format: Article
Language:English
Subjects:
Adolescent
Age Distribution
Biological and medical sciences
Biomarkers - blood
Brazil
Brazil - epidemiology
Child
Child, Preschool
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay
epidemiological survey
filter paper blood collection
hepatitis A
Hepatitis A - diagnosis
Hepatitis A - epidemiology
Hepatitis A - immunology
Hepatitis A Antibodies
Hepatitis Antibodies - blood
Human viral diseases
Humans
Infant
Infectious diseases
Medical sciences
Pilot Projects
Predictive Value of Tests
Prevalence
Sensitivity and Specificity
Tropical medicine
validation
Viral diseases
Viral hepatitis
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Summary:The validity of blood spotted on to filter-paper (BSOFP) eluates for the detection of antibodies against hepatitis A virus (HAV) was investigated in 718 individuals (children and adults) during a field study in a small area in Rio de Janeiro State, Brazil. Serum samples were considered the ‘gold standard’. BSOFP eluates were analysed by 2 different techniques: microplate competitive enzyme-linked immunosorbent assay (ELISA) of the whole study group and microparticle enzyme immune assay (MEIA) of a subsample of 59 individuals. For BSOFP eluates by ELISA, sensitivity and specificity were 89·6% (95% CI: 84·7–93·1) and 97·5% (95% CI: 95·6–98·7), respectively. For a seroprevalence of anti-HAV antibodies of 32%, the positive predictive value was 94·5% (95% CI: 90·3–97·0) and the negative predictive value was 95·2% (95% CI: 92·8–96·8). The test efficiency was 95·0% (95% CI: 93·1–96·4). Similar results were found for BSOFP eluates by MEIA. Agreement between the 2 techniques used for BSOFP (ELISA and MEIA) was also high (κ = 0·93). These results encourage the more widespread application of BSOFP as a means of surveillance for large-scale epidemiological studies for hepatitis A.
ISSN:0035-9203
1878-3503
DOI:10.1016/S0035-9203(99)90133-5