Crystallization and preliminary crystal analysis of yeast hexokinase PI and PII

Hexokinase is the prime enzyme of the Embden–Meyerhof pathway and is responsible for the first stage of energy conversion. It catalyzes the transfer of a phosphate to glucose to form glucose‐6‐phosphate. Yeast hexokinase PII is also known to play an important role in glucose signal transduction. Cry...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 1999-12, Vol.55 (12), p.2047-2048
Main Authors: Kuser, Paula R., Golubev, Alexander M., Polikarpov, Igor
Format: Article
Language:English
Subjects:
Crystallization
Crystallography, X-Ray
Hexokinase - chemistry
Hexokinase - isolation & purification
hexokinases
Isoenzymes - chemistry
Isoenzymes - isolation & purification
Saccharomyces cerevisiae - enzymology
transferases
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Summary:Hexokinase is the prime enzyme of the Embden–Meyerhof pathway and is responsible for the first stage of energy conversion. It catalyzes the transfer of a phosphate to glucose to form glucose‐6‐phosphate. Yeast hexokinase PII is also known to play an important role in glucose signal transduction. Crystals of yeast hexokinase isoforms PI and PII were obtained by vapour‐diffusion techniques using the hanging‐drop method. Isoform PI crystals belong to the space group P212121, with unit‐cell parameters a = 62.12, b = 78.87, c = 144.74 Å. Unit‐cell parameters for isoform PII crystals are a = b = 142.81, c = 58.46 Å and the space group is I4. Synchrotron diffraction data have been collected to 2.2 Å resolution from the isoform PII crystal, whereas isoform PI diffracted to 3.1 Å.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444999012263