Impaired function of CD4+/CD25+ T regulatory lymphocytes characterizes the self-limited hepatitis A virus infection

Background and Aim:  Hepatitis A virus (HAV) causes a transient illness leaving permanent protection against reinfection. Few data are available on the regulatory mechanisms involved in the CD4+ T helper activation. We aimed to investigate the frequency and function of CD3+/CD4+/CD25+ T cells with r...

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Published in:Journal of gastroenterology and hepatology 2008-07, Vol.23 (7pt2), p.e105-e110
Main Authors: Perrella, Alessandro, Vitiello, Laura, Atripaldi, Luigi, Sbreglia, Costanza, Grattacaso, Stella, Bellopede, Pasquale, Patarino, Tommaso, Morelli, Giuseppe, Altamura, Simona, Racioppi, Luigi, Perrella, Oreste
Format: Article
Language:English
Subjects:
Adult
anergy
Case-Control Studies
CD3 Complex - analysis
CD4 Antigens - analysis
CD4+/CD25
Cells, Cultured
Female
hepatitis
Hepatitis A - immunology
Hepatitis A virus
Hepatitis A Virus, Human - immunology
Humans
Interleukin-2 Receptor alpha Subunit - analysis
Lymphocyte Activation
Male
T-Lymphocytes, Regulatory - immunology
T-Lymphocytes, Regulatory - virology
Treg
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Summary:Background and Aim:  Hepatitis A virus (HAV) causes a transient illness leaving permanent protection against reinfection. Few data are available on the regulatory mechanisms involved in the CD4+ T helper activation. We aimed to investigate the frequency and function of CD3+/CD4+/CD25+ T cells with regulatory function (Tregs) during acute HAV infection. Methods:  We enrolled 35 consecutive patients and 15 healthy donors, enumerated Tregs by flow cytometry assay and evaluated, after immunomagnetical sorting with magnetic beads, their ability to inhibit the proliferation of CD4+/CD25– T lymphocytes at different ratios (1:1, 1:10, 1:20). Results:  All patients had the usual course of infection. Our immunological analysis showed Tregs frequency in these patients (6.5% [range, 5–8.8%]; 36 [range, 10–87] cells) did not have any statistical difference compared with healthy donors (6% [range, 5–8%]; 48 (range, 23–71) cells), while their ability to suppress CD4+/CD25– was drastically reduced at different ratios (Mann–Whitney U‐test; ratio 1:1, 93% vs 72%, z = −3.34, P 
ISSN:0815-9319
1440-1746
DOI:10.1111/j.1440-1746.2007.05008.x