Loading…

Metabolomic profiling of Drosophila using liquid chromatography Fourier transform mass spectrometry

Hydrophilic interaction chromatography (HILIC) interfaced with an Orbitrap Fourier transform mass spectrometer (FT-MS) was used to carry out metabolomic profiling of the classical Drosophila mutation, rosy ( ry). This gene encodes a xanthine oxidase/dehydrogenase. In addition to validating the techn...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 2008-08, Vol.582 (19), p.2916-2922
Main Authors: Kamleh, M.A., Hobani, Y., Dow, J.A.T., Watson, D.G.
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hydrophilic interaction chromatography (HILIC) interfaced with an Orbitrap Fourier transform mass spectrometer (FT-MS) was used to carry out metabolomic profiling of the classical Drosophila mutation, rosy ( ry). This gene encodes a xanthine oxidase/dehydrogenase. In addition to validating the technology by detecting the same changes in xanthine, hypoxanthine, urate and allantoin that have been reported classically, completely unsuspected changes were detected in each of the tryptophan, arginine, pyrimidine and glycerophospholipid metabolism pathways. The rosy mutation thus ramifies far more widely than previously detected.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2008.07.029