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Metabolomic profiling of Drosophila using liquid chromatography Fourier transform mass spectrometry
Hydrophilic interaction chromatography (HILIC) interfaced with an Orbitrap Fourier transform mass spectrometer (FT-MS) was used to carry out metabolomic profiling of the classical Drosophila mutation, rosy ( ry). This gene encodes a xanthine oxidase/dehydrogenase. In addition to validating the techn...
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Published in: | FEBS letters 2008-08, Vol.582 (19), p.2916-2922 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Hydrophilic interaction chromatography (HILIC) interfaced with an Orbitrap Fourier transform mass spectrometer (FT-MS) was used to carry out metabolomic profiling of the classical
Drosophila mutation,
rosy (
ry). This gene encodes a xanthine oxidase/dehydrogenase. In addition to validating the technology by detecting the same changes in xanthine, hypoxanthine, urate and allantoin that have been reported classically, completely unsuspected changes were detected in each of the tryptophan, arginine, pyrimidine and glycerophospholipid metabolism pathways. The rosy mutation thus ramifies far more widely than previously detected. |
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ISSN: | 0014-5793 1873-3468 |
DOI: | 10.1016/j.febslet.2008.07.029 |