The effects of substance P and vasoactive intestinal peptide on interleukin-6 synthesis in cultured human keratinocytes

Interleukin-6 (IL-6) is a cytokine implicated as a key mediator of immune and inflammatory responses in psoriasis. Recent studies have shown that neuropeptides, substance P (SP) and vasoactive intestinal peptide (VIP), can modulate a production of IL-6 from cells, such as monocytes and astrocytes, p...

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Bibliographic Details
Published in:Journal of dermatological science 1999-12, Vol.22 (1), p.17-23
Main Authors: Park, Y.M., Kim, C.W.
Format: Article
Language:English
Subjects:
Animals
Cattle
Cells, Cultured
Cultured human keratinocyte
Enzyme-Linked Immunosorbent Assay
Humans
Interleukin-6
Interleukin-6 - biosynthesis
Keratinocytes - metabolism
Substance P
Substance P - pharmacology
Vasoactive intestinal peptide
Vasoactive Intestinal Peptide - pharmacology
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Summary:Interleukin-6 (IL-6) is a cytokine implicated as a key mediator of immune and inflammatory responses in psoriasis. Recent studies have shown that neuropeptides, substance P (SP) and vasoactive intestinal peptide (VIP), can modulate a production of IL-6 from cells, such as monocytes and astrocytes, participating in an immune reaction. The aim of this study was to assess the role of the neuropeptides on cytokine production of keratinocytes in physiologic or pathologic conditions. Cultured human keratinocytes derived from normal foreskin and psoriatic lesions were treated with various concentrations of SP or VIP, in the presence or absence of fetal bovine serum. The secretion of IL-6 by the treated keratinocytes was analyzed by enzyme-linked immunosorbent assay. Although neither SP nor VIP, by itself, was able to induce IL-6 synthesis in cultured human keratinocytes, we have found that SP, not VIP, significantly reduced 5% fetal bovine serum-induced IL-6 production in time- and dose-dependent fashion. This down-regulatory effect of SP was reversed by spantide, a SP antagonist. Lesional psoriatic keratinocytes showed a similar, but weaker, response when compared with normal keratinocytes. These data suggested that SP might modulate IL-6 synthesis of keratinocytes in either physiologic or pathologic conditions such as psoriasis.
ISSN:0923-1811
1873-569X
DOI:10.1016/S0923-1811(99)00038-9