Proportion of tau protein to phosphorylated tau protein CSF levels in differential diagnosis of dementia

The diagnosis of Creutzfeldt-Jakob disease (CJD) is based on typical clinical features and can be supported by detection of 14-3-3 protein in the CSF. The present study suggests the importance of investigating this ratio of total tau protein to phosphorylated tau protein in differentiating CJD from...

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Bibliographic Details
Published in:Nervenarzt 2008-08, Vol.79 (8), p.891-898
Main Authors: Hort, J, Valis, M, Waberzinek, G, Taláb, R, Glossová, L, Bojar, M, Vyhnálek, M, Skoda, D, Masopust, J, Stourac, P
Format: Article
Language:ger
Subjects:
14-3-3 Proteins - cerebrospinal fluid
Aged
Alzheimer Disease - blood
Alzheimer Disease - cerebrospinal fluid
Creutzfeldt-Jakob Syndrome - blood
Creutzfeldt-Jakob Syndrome - cerebrospinal fluid
Dementia - blood
Dementia - cerebrospinal fluid
Dementia - diagnosis
Diagnosis, Differential
Female
Humans
Male
Middle Aged
Phosphorylation
Reproducibility of Results
Sensitivity and Specificity
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Summary:The diagnosis of Creutzfeldt-Jakob disease (CJD) is based on typical clinical features and can be supported by detection of 14-3-3 protein in the CSF. The present study suggests the importance of investigating this ratio of total tau protein to phosphorylated tau protein in differentiating CJD from other dementias. Thirty-one patients with Alzheimer's disease (AD) or frontotemporal dementia and four with definitive diagnoses of CJD were included in the study. Results from baseline investigations were compared with those from an age-matched cognitively controlled group with Bell's palsy. Tau protein, phosphorylated tau protein, and beta amyloid were analyzed using a commercially available enzyme-linked immunosorbent assay; 14-3-3 protein was assessed by Western blotting. A distinctly high proportion of total tau protein to phosphorylated tau protein in CSF was found in all patients diagnosed with CJD, even in those with negative 14-3-3 protein blot results. In contrast, marker analysis in patients with Alzheimer's dementia revealed the highest CSF ratio of beta amyloid to phosphorylated tau protein levels. These proteins are important diagnostic biomarkers for CJD, especially in patients with negative 14-3-3 protein findings.
ISSN:0028-2804
1433-0407
DOI:10.1007/s00115-008-2501-7