Interference of aluminium on iron metabolism in erythroleukaemia K562 cells

It has been suggested that aluminium (Al) has a deleterious effect on erythropoiesis. However, there is still uncertainty as to its action mechanism. The present work was designed to determine how Al could affect the iron (Fe) metabolism in the human erythroleukaemia cell line K562. These cells, tha...

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Bibliographic Details
Published in:Journal of inorganic biochemistry 1999-08, Vol.76 (2), p.105-112
Main Authors: Pérez, Gladys, Garbossa, Graciela, Sassetti, Beatriz, Di Risio, Cecilia, Nesse, Alcira
Format: Article
Language:English
Subjects:
Aluminium toxicity
aluminum
Aluminum - pharmacology
Cell Differentiation - drug effects
cell lines
Erythroid differentiation
Haemoglobin synthesis
Humans
Iodine Radioisotopes
Iron - metabolism
K562 cell line
K562 Cells
Leukemia, Erythroblastic, Acute - metabolism
Leukemia, Erythroblastic, Acute - pathology
Radioligand Assay
Receptors, Transferrin - metabolism
toxicity
Transferrin - metabolism
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Summary:It has been suggested that aluminium (Al) has a deleterious effect on erythropoiesis. However, there is still uncertainty as to its action mechanism. The present work was designed to determine how Al could affect the iron (Fe) metabolism in the human erythroleukaemia cell line K562. These cells, that express surface transferrin receptors (TfRs), were induced to erythroid differentiation by either haemin or hydroxyurea in 72 h cultures in media containing apotransferrin (apoTf). In the presence of aluminium citrate, the number of benzidine-positive cells decreased 18% when the cultures were induced by haemin, and 30% when hydroxyurea was the inducer. Cell viability was always unaffected. From competition assays, surface binding of 125I–Tf–Fe 2 was found to be inversely related ( p
ISSN:0162-0134
1873-3344
DOI:10.1016/S0162-0134(99)00121-X