Preparation of hydroxyapatite-nanocrystal-coated stainless steel, and its cell interaction

Calcined nanocrystals of hydroxyapatite (HAp) having spherical or rod‐shaped morphologies were coated through covalent linkage on a type 316L stainless steel substrate, which was chemically modified by the graft polymerization of γ‐methacryloxypropyl triethoxysilane (MPTS) at 70–110°C. The grafting...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2008-09, Vol.86A (3), p.589-596
Main Authors: Okada, Masahiro, Masuda, Miwa, Tanaka, Ryoichi, Miyatake, Kunio, Kuroda, Daisuke, Furuzono, Tsutomu
Format: Article
Language:English
Subjects:
Cell Adhesion
Cell Communication
Cells, Cultured
composite
covalent linkage
Durapatite - metabolism
Humans
hydroxyapatite
Materials Testing
nano-sized crystal
Nanoparticles - ultrastructure
Silanes - metabolism
Spectroscopy, Fourier Transform Infrared
Spectrum Analysis
Stainless Steel
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Summary:Calcined nanocrystals of hydroxyapatite (HAp) having spherical or rod‐shaped morphologies were coated through covalent linkage on a type 316L stainless steel substrate, which was chemically modified by the graft polymerization of γ‐methacryloxypropyl triethoxysilane (MPTS) at 70–110°C. The grafting of poly(MPTS) on the substrate was confirmed by X‐ray photoelectron spectroscopy (XPS) and attenuated total reflection Fourier transform infrared spectroscopy (ATR FT‐IR). In order to coat the substrate with the HAp crystals through covalent linkage, the reactionbetween the alkoxysilyl groupsin the poly (MPTS) grafted on the substrate and the OH groups on the HAp crystals was conducted at 80°C. The poly(MPTS)‐grafted substrate was strongly coated with the HAp nanocrystals, although the HAp crystals adsorbed physically on the original substrate without poly(MPTS) grafting were removed by ultrasonic treatment. Human umbilical vein endothelial cells (HUVEC) adhered in larger numbers on the HAp‐coated stainless steel substrate as compared with the original substrate after 24 h of initial incubation. The number of HUVEC adhered on the rod‐shaped HAp‐coated substrate was not significantly different from that on the spherical HAp‐coated substrate under the present conditions. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.31668