Chondrocytes harvested from osteochondritis dissecans cartilage are able to undergo limited in vitro chondrogenesis despite having perturbations of cell phenotype in vivo

Our objective was to characterize the variation in gene expression for key genes associated with chondrogenic phenotype of osteochondrosis (OC)‐affected and normal chondrocytes, and to identify whether OC chondrocytes can redifferentiate and regain a phenotype similar to normal chondrocytes if appro...

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Bibliographic Details
Published in:Journal of orthopaedic research 2008-08, Vol.26 (8), p.1133-1140
Main Authors: Garvican, E.R., Vaughan-Thomas, A., Redmond, C., Clegg, P.D.
Format: Article
Language:English
Subjects:
Animals
Cartilage - cytology
Cartilage - physiology
Cell Differentiation - physiology
Cells, Cultured
Chondrocytes - pathology
Chondrocytes - physiology
chondrogenesis
Chondrogenesis - physiology
Collagen - genetics
Extracellular Matrix Proteins - genetics
gene expression
Gene Expression - physiology
Horse Diseases - pathology
Horse Diseases - physiopathology
Horses
In Vitro Techniques
Matrix Metalloproteinases - genetics
Osteoarthritis - pathology
Osteoarthritis - physiopathology
Osteoarthritis - veterinary
Osteochondritis Dissecans - pathology
Osteochondritis Dissecans - physiopathology
Osteochondritis Dissecans - veterinary
osteochondrosis
Phenotype
Signal Transduction - physiology
Tissue Inhibitor of Metalloproteinases - genetics
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Summary:Our objective was to characterize the variation in gene expression for key genes associated with chondrogenic phenotype of osteochondrosis (OC)‐affected and normal chondrocytes, and to identify whether OC chondrocytes can redifferentiate and regain a phenotype similar to normal chondrocytes if appropriate chondrogenic signals are given. Equine articular cartilage removed at surgery to treat clinically significant OC lesions was collected (n = 10), and the gene expression evaluated and compared to aged‐matched normal samples (n = 10). Cartilage was harvested from normal (n = 4) and OC (n = 3) joints from horses at necropsy. Chondrogenic pellet cultures were established following monolayer proliferation. After 14 days in culture, the pellets were assessed by histochemical and pellet weight analysis, assay of glycosaminoglycan (GAG) content, and gene expression. Chondrocytes from OC cartilage expressed significantly more Coll‐I, ‐II, ‐III, and ‐X than chondrocytes from normal cartilage (all p 
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.20602