Antioxidant status and glutathione metabolism in peripheral blood mononuclear cells from patients with chronic hepatitis C

Background/Aims: Oxidative stress could play a role in the pathogenesis of hepatitis C virus infection. We investigated the oxidant/antioxidant status in peripheral blood mononuclear cells from patients with chronic hepatitis C and controls. Methods/Results: Lipid peroxidation products and superoxid...

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Published in:Journal of hepatology 1999-11, Vol.31 (5), p.808-814
Main Authors: Boya, Patricia, Peña, Andrés de la, Beloqui, Oscar, Larrea, Esther, Conchillo, Marian, Castelruiz, Yurdana, Civeira, María-Pilar, Prieto, Jesús
Format: Article
Language:English
Subjects:
Adult
Aged
Antioxidant enzymes
Antioxidants - metabolism
Bacterial diseases
Bacterial diseases of the urinary system
Biological and medical sciences
Catalase - blood
Cohort Studies
Cytosol - metabolism
Female
Glutamate-Cysteine Ligase - blood
Glutamate-Cysteine Ligase - genetics
Glutathione
Glutathione - blood
Glutathione Peroxidase - blood
Glutathione Reductase - blood
Hepatitis C virus
Hepatitis C, Chronic - blood
Human bacterial diseases
Humans
Infectious diseases
Leukocytes, Mononuclear - metabolism
Lipid Peroxidation
Male
Malondialdehyde
Malondialdehyde - blood
Medical sciences
Middle Aged
Oxidative stress
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Summary:Background/Aims: Oxidative stress could play a role in the pathogenesis of hepatitis C virus infection. We investigated the oxidant/antioxidant status in peripheral blood mononuclear cells from patients with chronic hepatitis C and controls. Methods/Results: Lipid peroxidation products and superoxide dismutase activity in peripheral blood mononuclear cells were higher in chronic hepatitis C patients than in healthy subjects while glutathione S-transferase activity was reduced in patients as compared to controls. Catalase, glutathione peroxidase and glutathione reductase were similar in chronic hepatitis C and normal individuals. No statistically significant differences were found between patients and controls with regard to glutathione levels in peripheral blood mononuclear cells, but 35% of patients with chronic hepatitis C showed values of glutathione and oxidized glutathione which were below and above, respectively, the limits of normal controls. Finally, the glutathione synthetic capacity of the cytosol of peripheral blood mononuclear cells was significantly higher in patients than in controls, indicating increased glutathione turnover in lymphocytes from patients with chronic hepatitis C. Conclusions: Oxidative stress is observed in peripheral blood mononuclear cells from chronic hepatitis C patients. This process might alter lymphocyte function and facilitate the chronicity of the infection.
ISSN:0168-8278
1600-0641
DOI:10.1016/S0168-8278(99)80281-5