Identification of a single-nucleocapsid baculovirus isolated from Clanis bilineata tsingtauica (Lepidoptera: Sphingidae)

A nucleopolyhedrovirus isolated from infected larvae of Clanis bilineata tsingtauica was characterized. Electron microscopical studies on the ultrastructure of C. bilineata nucleopolyhedrovirus (ClbiSNPV) occlusion bodies (OBs) showed several virions (up to 16) with a single nucleocapsid packaged wi...

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Bibliographic Details
Published in:Archives of virology 2008-08, Vol.153 (8), p.1557-1561
Main Authors: Wang, Liqun, Yi, Jianping, Zhu, Shanying, Li, Bing, Chen, Yan, Shen, Weide, Wang, Wenbing
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cloning
DNA, Viral - chemistry
DNA, Viral - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Viral
Insects
Larva - virology
Lepidoptera - virology
Life sciences
Microbiology
Miscellaneous
Nucleocapsid - genetics
Nucleopolyhedrovirus - classification
Nucleopolyhedrovirus - genetics
Nucleopolyhedrovirus - isolation & purification
Pesticides
Phylogenetics
Viral Proteins - chemistry
Viral Proteins - genetics
Virology
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Summary:A nucleopolyhedrovirus isolated from infected larvae of Clanis bilineata tsingtauica was characterized. Electron microscopical studies on the ultrastructure of C. bilineata nucleopolyhedrovirus (ClbiSNPV) occlusion bodies (OBs) showed several virions (up to 16) with a single nucleocapsid packaged within a single viral envelope. The diameter of the OBs was 0.77-1.7 μm with a mean of 1.13 ± 0.19 μm. The complete sequence of the ClbiSNPV polyhedrin (polh) gene contained 741 nucleotides, predicting a protein of 246 amino acids. Phylogenetic analyses using the complete sequence of the polh genes indicated that ClbiSNPV clusters with Group II NPVs. This is the first record of a baculovirus from C. bilineata.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-008-0153-3