Simultaneous multifocal, multiphoton, photon counting microscopy

We demonstrate a novel multifocal, multiphoton microscope that is capable of simultaneous dynamic imaging of multiple focal planes. We show for the first time that multimodal, multiphoton images excited with orthogonal polarizations can be acquired simultaneously in both the transmission and epi dir...

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Bibliographic Details
Published in:Optics express 2008-07, Vol.16 (14), p.10364-10371
Main Authors: Carriles, Ramón, Sheetz, Kraig E, Hoover, Erich E, Squier, Jeff A, Barzda, Virginijus
Format: Article
Language:English
Subjects:
Algorithms
Equipment Design
Image Processing, Computer-Assisted
Lasers
Light
Microscopy - instrumentation
Microscopy - methods
Microscopy, Fluorescence, Multiphoton - instrumentation
Microscopy, Fluorescence, Multiphoton - methods
Optics and Photonics
Photons
Signal Processing, Computer-Assisted
Time Factors
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Description
Summary:We demonstrate a novel multifocal, multiphoton microscope that is capable of simultaneous dynamic imaging of multiple focal planes. We show for the first time that multimodal, multiphoton images excited with orthogonal polarizations can be acquired simultaneously in both the transmission and epi directions.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.16.010364