Somatostatin receptor subtype 2A expression in the rat retina

Somatostatin is mainly expressed by sparsely occurring amacrine and interplexiform cells in the retina. In this study, we characterized the expression and cellular localization of one of the somatostatin subtype (sst) receptors, sst 2A, in the rat retina. The presence of sst 2A receptor messenger RN...

Full description

Saved in:
Bibliographic Details
Published in:Neuroscience 1999-01, Vol.94 (3), p.675-683
Main Authors: Johnson, J., Wu, V., Wong, H., Walsh, J.H., Brecha, N.C.
Format: Article
Language:English
Subjects:
amacrine cells
Animals
Biological and medical sciences
bipolar cells
Eye and associated structures. Visual pathways and centers. Vision
Female
Fundamental and applied biological sciences. Psychology
horizontal cells
Male
neuropeptide receptors
photoreceptors
Rats
Rats, Sprague-Dawley
Receptors, Somatostatin - analysis
Receptors, Somatostatin - genetics
Restriction Mapping
Retina - cytology
Retina - metabolism
Retinal Rod Photoreceptor Cells - cytology
Retinal Rod Photoreceptor Cells - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Tyrosine 3-Monooxygenase - analysis
Vertebrates: nervous system and sense organs
visual system
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Somatostatin is mainly expressed by sparsely occurring amacrine and interplexiform cells in the retina. In this study, we characterized the expression and cellular localization of one of the somatostatin subtype (sst) receptors, sst 2A, in the rat retina. The presence of sst 2A receptor messenger RNA in retinal extracts was demonstrated by reverse transcription–polymerase chain reaction using specific primers to detect the sst 2 receptor and its isoforms, sst 2A and sst 2B. Specific sst 2A receptor immunoreactivity was mainly localized to the plasma membrane of several neuronal cell types. In the outer retina, immunoreactivity was localized to cone photoreceptors, horizontal cells, and rod and cone bipolar cells. Double-label experiments showed the co-localization of sst 2A receptor and protein kinase C (α and β), a rod bipolar cell marker, and of sst 2A receptor and Calbindin-D 28k, a horizontal cell marker. In the inner retina, sst 2A receptor immunoreactivity occurred in tyrosine hydroxylase-positive amacrine cells; most were of medium to large size. These findings indicate that somatostatin may act at a distance, in a paracrine manner, on several cell types that express the sst 2A receptor, and therefore exert a broad modulatory influence on both scotopic and photopic visual pathways.
ISSN:0306-4522
1873-7544
DOI:10.1016/S0306-4522(99)00170-0