Strategies for the Storage of Ancylostoma caninum Third-Stage Larvae

Although cryopreservation protocols for storage of hookworm larvae have been described, the circumstances under which the technique is necessary to ensure larval survival are not well defined. The motility of infective-stage larvae (as judged by observation) and their ability to migrate through cani...

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Bibliographic Details
Published in:The Journal of parasitology 2008-06, Vol.94 (3), p.755-756
Main Authors: Kopp, S. R, Kotze, A. C, McCarthy, J. S, Morton, J. M, Coleman, G. T
Format: Article
Language:English
Subjects:
Ancylostoma - physiology
Animals
Biological and medical sciences
Canines
Cryopreservation
Cryopreservation - methods
Cryopreservation - standards
Cryopreservation - veterinary
Culture Media
Dogs
Experiments
Feces - parasitology
Flasks
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Glycerol
Infective stages
Invertebrates
Larva - physiology
Larvae
Motility
Nematode larvae
Nitrogen
Parasites
Preservation, Biological - methods
Preservation, Biological - standards
Preservation, Biological - veterinary
Proportions
Research Notes
Room temperature
Storage conditions
Temperature
Worms
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Summary:Although cryopreservation protocols for storage of hookworm larvae have been described, the circumstances under which the technique is necessary to ensure larval survival are not well defined. The motility of infective-stage larvae (as judged by observation) and their ability to migrate through canine skin in vitro were measured over a 7-mo period in worms held at room temperature and worms that had been cryopreserved at the start of the experiment. Cryopreserved worms showed motility and migration proportions of 45.6–48.0% and 26.8– 34.0%, respectively, throughout the experiment, compared with percentages of 92.7 and 84.1%, respectively, in the original fresh worms. Larvae held at room temperature showed a gradual decrease in motility and migration ability over the experimental period. Motility and migratory ability of cryopreserved larvae was only significantly higher (P < 0.01) than room temperature-stored larvae from 4 and 5 mo onward, respectively.
ISSN:0022-3395
1937-2345
DOI:10.1645/GE-1301.1