Mapping of the conserved antigenic domains shared between potato apyrase and parasite ATP diphosphohydrolases: potential application in human parasitic diseases

Evolutionary and closer structural relationships are demonstrated by phylogenetic analysis, peptide prediction and molecular modelling between Solanum tuberosum apyrase, Schistosoma mansoni SmATPase 2 and Leishmania braziliensis NDPase. Specific protein domains are suggested to be potentially involv...

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Bibliographic Details
Published in:Parasitology 2008-07, Vol.135 (8), p.943-953
Main Authors: FARIA-PINTO, P., REZENDE-SOARES, F. A., MOLICA, A. M., MONTESANO, M. A., MARQUES, M. J., ROCHA, M. O. C., GOMES, J. A. S., ENK, M. J., CORREA-OLIVEIRA, R., COELHO, P. M. Z., NETO, S. M., FRANCO, O. L., VASCONCELOS, E. G.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animal, plant and microbial ecology
Animals
Antibodies, Protozoan - immunology
antibody
apyrase
Apyrase - chemistry
Apyrase - immunology
ATP diphosphohydrolase
Biological and medical sciences
Chagas Disease - blood
Chagas Disease - immunology
Conserved Sequence - immunology
Epitope Mapping
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
General aspects. Techniques
Humans
Invertebrates
Leishmania braziliensis
Leishmania braziliensis - enzymology
Leishmania braziliensis - genetics
Leishmania braziliensis - immunology
Leishmaniasis, Cutaneous - blood
Leishmaniasis, Cutaneous - immunology
Methods and techniques (sampling, tagging, trapping, modelling...)
molecular modelling
Molecular Sequence Data
NTPDase
Parasites - enzymology
Parasites - genetics
Parasites - immunology
phylogenetic
Phylogeny
Protein Structure, Tertiary
Schistosoma mansoni
Schistosoma mansoni - enzymology
Schistosoma mansoni - genetics
Schistosoma mansoni - immunology
Schistosomiasis - blood
Schistosomiasis - immunology
Sequence Alignment
Solanum tuberosum
Solanum tuberosum - enzymology
Trypanosoma cruzi
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Summary:Evolutionary and closer structural relationships are demonstrated by phylogenetic analysis, peptide prediction and molecular modelling between Solanum tuberosum apyrase, Schistosoma mansoni SmATPase 2 and Leishmania braziliensis NDPase. Specific protein domains are suggested to be potentially involved in the immune response, and also seem to be conserved during host and parasite co-evolution. Significant IgG antibody reactivity was observed in sera from patients with American cutaneous leishmaniasis (ACL) and schistosomiasis using potato apyrase as antigen in ELISA. S. mansoni adult worm or egg, L. braziliensis promastigote (Lb) and Trypanosoma cruzi epimastigote (EPI) have ATP diphosphohydrolases, and antigenic preparations of them were evaluated. In ACL patients, IgG seropositivity was about 43% and 90% for Lb and potato apyrase, respectively, while IgM was lower (40%) or IgG (100%) seropositivity for both soluble egg (SEA) and adult worm (SWAP) antigens was higher than that found for potato apyrase (IgM=10%; IgG=39%). In Chagas disease, IgG seropositivity for EPI and potato apyrase was 97% and 17%, respectively, while the IgM was low (3%) for both antigens. The study of the conserved domains from both parasite proteins and potato apyrase could lead to the development of new drug targets or molecular markers.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182008004538