Glycoprotein Derived from the Hot Water Extract of Mint Plant, Perilla frutescens Britton

Glycoprotein showing inhibitory activity against mast cell degranulation and hyaluronidase activity was purified from the hot water extract of mint plant (Perilla frutescens Britton). The purified inhibitor gave a single band detected with Coomassie brilliant blue staining and periodic acid-Schiff s...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1999-02, Vol.47 (2), p.468-472
Main Authors: Asada, Masahiro, Fukumori, Yasunori, Inoue, Mami, Nakagomi, Kazuya, Sugie, Makiko, Fujita, Yasuko, Tomizuka, Noboru, Yamazaki, Yoshimitsu, Oka, Syuichi
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Degranulation - drug effects
characterization
enzyme inhibitors
Enzyme Inhibitors - isolation & purification
Enzyme Inhibitors - pharmacology
General pharmacology
glycoproteins
Glycoproteins - isolation & purification
Glycoproteins - pharmacology
hyaluronoglucosaminidase
Hyaluronoglucosaminidase - antagonists & inhibitors
In Vitro Techniques
inactivation
inhibitors
isolation
Lamiaceae - chemistry
leaves
mast cell degranulation inhibitors
mast cells
Mast Cells - drug effects
Mast Cells - ultrastructure
Medical sciences
Perilla frutescens
Pharmacognosy. Homeopathy. Health food
pharmacology
Pharmacology. Drug treatments
plant extracts
Plant Extracts - analysis
Plant Leaves - chemistry
protein kinase C
Protein Kinase C - antagonists & inhibitors
Rats
Rats, Wistar
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Summary:Glycoprotein showing inhibitory activity against mast cell degranulation and hyaluronidase activity was purified from the hot water extract of mint plant (Perilla frutescens Britton). The purified inhibitor gave a single band detected with Coomassie brilliant blue staining and periodic acid-Schiff staining on sodium dodecyl sulfate−polyacrylamide gel electrophoresis (SDS−PAGE) analysis. The molecular mass was estimated to be 6.0 kDa on SDS−PAGE. The inhibitor did not become inactivated when boiled for 30 min or digested with trypsin, V8 protease, or proteinase K but was inactivated by NaIO4 oxidation. The inhibitor prevented mast cell degranulation and hyaluronidase activity (IC50 = 0.42 mg/mL) in a dose-dependent manner. The inhibitor also inhibited the protein kinase C activity. It is possible to purify and characterize a glycoprotein with putative pharmacological properties from mint plants. Keywords: Mint plant; Perilla frutescens Britton; glycoprotein; inhibitor; hyaluronidase; mast cell degranulation; protein kinase C
ISSN:0021-8561
1520-5118
DOI:10.1021/jf9802777