Constituents of glandular trichomes of Tithonia diversifolia: Relationships to herbivory and antifeedant activity

The herbivory activity of the bordered patch larvae ( Chlosyne lacinia) on leaves of a Brazilian population of Tithonia diversifolia and the antifeedant potential of its leaf rinse extract were investigated. Our findings indicate that the caterpillars avoid the sesquiterpene-lactone-rich glandular t...

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Bibliographic Details
Published in:Phytochemistry (Oxford) 2008-07, Vol.69 (10), p.2052-2060
Main Authors: Ambrósio, Sérgio Ricardo, Oki, Yumi, Heleno, Vladimir Constantino Gomes, Chaves, Juliana Siqueira, Nascimento, Paulo Gustavo Barboni Dantas, Lichston, Juliana Espada, Constantino, Mauricio Gomes, Varanda, Elenice Mouro, Da Costa, Fernando Batista
Format: Article
Language:English
Subjects:
Animals
Antifeedant activity
antifeeding activity
Asteraceae
Asteraceae - chemistry
Biological and medical sciences
chemical constituents of plants
Chemical constitution
Chemical control
Chlosyne lacinia
Control
diterpenoids
Eating - drug effects
Eating - physiology
flavonoids
Fundamental and applied biological sciences. Psychology
Glandular trichomes
herbivores
Herbivory activity
insect pests
Insecta - drug effects
Insecta - physiology
Larva - drug effects
Magnetic Resonance Spectroscopy
Molecular Structure
Nymphalidae
Phytopathology. Animal pests. Plant and forest protection
phytophagous insects
Plant Extracts - chemistry
Plant Extracts - pharmacology
Plant Leaves - chemistry
Plant physiology and development
plant-insect relations
Protozoa. Invertebrates
Seasons
Sesquiterpene lactones
sesquiterpenoid lactones
Tithonia diversifolia
trichomes
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Summary:The herbivory activity of the bordered patch larvae ( Chlosyne lacinia) on leaves of a Brazilian population of Tithonia diversifolia and the antifeedant potential of its leaf rinse extract were investigated. Our findings indicate that the caterpillars avoid the sesquiterpene-lactone-rich glandular trichomes, and provide evidence for the antifeedant activity of this class of compounds. The herbivory activity of the bordered patch larvae ( Chlosyne lacinia, Lepidoptera) on leaves of a Brazilian population of Tithonia diversifolia and the antifeedant potential of its leaf rinse extract were investigated. The caterpillars fed only on the adaxial face, where the density of glandular trichomes is very low, and avoided the abaxial face, which contains high levels of trichomes. Deterrent activity against the larvae was observed in leaf discs treated with leaf rinse extract at concentrations of 1–5% of fresh leaf weight. High-performance liquid chromatography (HPLC) analysis indicated that sesquiterpene lactones are the main constituents of the glandular trichomes. Dichloromethane rinse extracts of the leaves and inflorescences were chemically investigated, and 16 compounds were isolated and identified: 14 sesquiterpene lactones, a flavonoid and a diterpenoid. In this study, five sesquiterpene lactones are described for the first time in the genus, including two lactones, one of which has an unusual seco-guaianolide skeleton. Our findings indicate that the caterpillars avoid the sesquiterpene-lactone-rich glandular trichomes, and provide evidence for the antifeedant activity of the dichloromethane leaf rinse extract. In addition, a study of the seasonal variation of the main constituents from the leaf surface throughout a year demonstrated that a very low qualitative but a very high quantitative variation occurs. The highest level of the main metabolite tagitinin C was observed between September and October and the lowest was from March to June, the later corresponding to the period of highest infestation by the larvae.
ISSN:0031-9422
1873-3700
DOI:10.1016/j.phytochem.2008.03.019