Single Molecule Fluorescence of Native and Refolded Peridinin–Chlorophyll–Protein Complexes

Single molecule spectroscopy was applied to study the optical properties of native and refolded peridinin–chlorophyll–protein (PCP) complexes. The native system is a trimer with six chlorophyll a (Chl a ) molecules, while the refolded one contains two Chl a and resembles structurally and spectroscop...

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Bibliographic Details
Published in:Journal of fluorescence 2008-07, Vol.18 (3-4), p.611-617
Main Authors: Wörmke, Stephan, Mackowski, Sebastian, Schaller, Andreas, Brotosudarmo, Tatas H. P., Johanning, Silke, Scheer, Hugo, Bräuchle, Christoph
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biomedicine
Biophysics
Biotechnology
Buffers
Carotenoids - chemistry
Carotenoids - genetics
Chlorophyll - chemistry
Fluorescence Polarization
Light-Harvesting Protein Complexes - chemistry
Light-Harvesting Protein Complexes - genetics
Original Paper
Peptide Fragments - genetics
Polyvinyl Alcohol - chemistry
Protein Conformation
Protozoan Proteins - chemistry
Protozoan Proteins - genetics
Recombinant Proteins - chemistry
Solvents - chemistry
Spectrometry, Fluorescence
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Summary:Single molecule spectroscopy was applied to study the optical properties of native and refolded peridinin–chlorophyll–protein (PCP) complexes. The native system is a trimer with six chlorophyll a (Chl a ) molecules, while the refolded one contains two Chl a and resembles structurally and spectroscopically the PCP monomer. The fluorescence emission of single PCP complexes strongly broadens with increasing excitation power. Simultaneously, the distribution of fluorescence maximum frequencies is also broadened. These spectral changes are attributed to photoinduced conformational changes of the protein that influence the fluorescence of embedded chromophores. Comparison of fluorescence intensities measured for PCP complexes in two different solvents indicates that the native PCP trimers are preserved in EDTA Tris buffer, while in PVA polymer matrix only monomers are stable.
ISSN:1053-0509
1573-4994
DOI:10.1007/s10895-008-0310-9