Physical interaction between Tbx6 and mespb is indispensable for the activation of bowline expression during Xenopus somitogenesis

During vertebrate somitogenesis, various transcriptional factors function coordinately to determine the position of the somite boundary. Previously, we reported on the signaling crosstalk that occurs between two major transcription factors involved in somitogenesis, Tbx6 and mespb/mesp2. These facto...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-08, Vol.372 (4), p.607-612
Main Authors: Hitachi, Keisuke, Danno, Hiroki, Kondow, Akiko, Ohnuma, Kiyoshi, Uchiyama, Hideho, Ishiura, Shoichi, Kurisaki, Akira, Asashima, Makoto
Format: Article
Language:English
Subjects:
Animals
Basic Helix-Loop-Helix Transcription Factors - genetics
Basic Helix-Loop-Helix Transcription Factors - metabolism
Bowline
Gene Expression Regulation, Developmental
Mesp2
Mespb
Physical interaction
Presomitic mesoderm
Protein Interaction Domains and Motifs
Protein Interaction Mapping
Regulatory Elements, Transcriptional
Ripply2
Segmentation
Somites - embryology
Somites - metabolism
Somitogenesis
T-Box Domain Proteins - metabolism
Tbx6
Xenopus
Xenopus laevis
Xenopus laevis - embryology
Xenopus laevis - genetics
Xenopus laevis - metabolism
Xenopus Proteins - genetics
Xenopus Proteins - metabolism
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Summary:During vertebrate somitogenesis, various transcriptional factors function coordinately to determine the position of the somite boundary. Previously, we reported on the signaling crosstalk that occurs between two major transcription factors involved in somitogenesis, Tbx6 and mespb/mesp2. These factors synergistically activated the expression of a downstream gene, bowline/Ripply2, which is essential for precise formation of the somite boundary. However, the molecular mechanism underlying this synergistic effect remains unclear. In this report, we found that the Tbx6 and mespb proteins interacted physically with each other. Pulldown assays with various deletion mutants of these proteins identified the essential domains for this physical interaction. Finally, we found that interference with the physical interaction by a dominant-negative form of mespb, mespbΔDBD, abrogated the expression of the bowline gene during Xenopus somitogenesis. These results indicate that the appropriate expression of bowline/Ripply2 is regulated by a direct interaction between the Tbx6 and mespb proteins during Xenopus somitogenesis.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.05.083