Bacterial leakage versus dye leakage in obturated root canals

Aim The aim of this study was to compare in vitro bacterial and dye leakage tests, commonly used to determine the seal of root canal fillings. Methodology Ninety‐six single‐rooted teeth had their crowns removed at the cemento‐enamel junction and their roots instrumented to ISO size 60 within 1 mm of...

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Bibliographic Details
Published in:International endodontic journal 1999-09, Vol.32 (5), p.370-375
Main Authors: Barthel, C. R., Moshonov, J., Shuping, G., Òrstavik, D.
Format: Article
Language:English
Subjects:
bacterial leakage
Bismuth
Coloring Agents
Dental Leakage - diagnosis
Dentistry
Drug Combinations
dye leakage
Epoxy Resins
Evaluation Studies as Topic
Glass Ionomer Cements
Humans
Methenamine
root canal filling
Root Canal Filling Materials
Root Canal Obturation
Rosaniline Dyes
Silver
Titanium
Zinc Oxide-Eugenol Cement
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Summary:Aim The aim of this study was to compare in vitro bacterial and dye leakage tests, commonly used to determine the seal of root canal fillings. Methodology Ninety‐six single‐rooted teeth had their crowns removed at the cemento‐enamel junction and their roots instrumented to ISO size 60 within 1 mm of the apex. Three groups of 30 roots were obturated by lateral condensation using gutta‐percha and one or other of the sealers, AH26, Ketac Endo, or Roth's 801 sealer. Three roots were used as negative controls and three roots as positive controls. The roots were then exposed at their coronal end first for 38 days to soy broth containing Staphylococcusepidermidis and thereafter for 48 h to basic fuchsin. Bacterial leakage was recorded when the challenging organism could be recovered from the apical end. Dye leakage was checked by microscopy of transverse sections of the apical tip at the end of the experiment. Results For the bacterial experiment, there was no significant difference amongst the three sealer groups. The dye experiment showed significantly greater leakage in the AH26 compared to the Ketac Endo group. No correlation between the results of the two tests could be seen. Conclusions The results of this study suggest that the molecular size of the penetrating agent may not be the relevant parameter when attempting to determine an appropriate test for the sealability of root canal fillings.
ISSN:0143-2885
1365-2591
DOI:10.1046/j.1365-2591.1999.00235.x