Differential responses of human monocytes and macrophages to IL-4 and IL-13

The primary interleukin‐4 (IL‐4) receptor complex on monocytes (type I IL‐4 receptor) includes the 140‐kDa α chain (IL‐4Rα) and the IL‐2 receptor γ chain, γc, which heterodimerize for intracellular signaling, resulting in suppression of lipopolysaccharide (LPS)‐inducible inflammatory mediator produc...

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Bibliographic Details
Published in:Journal of leukocyte biology 1999-10, Vol.66 (4), p.575-578
Main Authors: Hart, Prue H., Bonder, Claudine S., Balogh, Julianna, Dickensheets, Harold L., Donnelly, Raymond P., Finlay‐Jones, John J.
Format: Article
Language:English
Subjects:
a chain
Cell Differentiation
Humans
inflammatory cells
Interleukin-13 - immunology
Interleukin-13 Receptor alpha1 Subunit
Interleukin-4 - immunology
Macrophages - immunology
Monocytes - cytology
Monocytes - immunology
Receptors, Interleukin - immunology
Receptors, Interleukin-13
Receptors, Interleukin-2 - immunology
Receptors, Interleukin-4 - immunology
γ chain
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Summary:The primary interleukin‐4 (IL‐4) receptor complex on monocytes (type I IL‐4 receptor) includes the 140‐kDa α chain (IL‐4Rα) and the IL‐2 receptor γ chain, γc, which heterodimerize for intracellular signaling, resulting in suppression of lipopolysaccharide (LPS)‐inducible inflammatory mediator production. The activity of IL‐13 on human monocytes is very similar to that of IL‐4 because the predominant signaling chain (IL‐4Rα) is common to both receptors. In fact, IL‐4Rα with IL‐13Rα1 is designated both as an IL‐13 receptor and the type II IL‐4 receptor. When the anti‐inflammatory activities of IL‐4 and IL‐13 were investigated on synovial fluid macrophages and compared with the responses by monocytes isolated from the patients at the same time as joint drainage, the response profiles differed with some responses similar in the two cell populations, others reduced on the inflammatory cells. Similar differences were recorded in the response profiles to IL‐4 and IL‐13 by monocytes and monocytes cultured for 7 days in macrophage colony‐stimulating factor (M‐CSF) or granulocyte‐macrophage CSF (GM‐CSF) (monocyte‐derived macrophages, MDMac). MDMac have reduced γc mRNA levels and reduced expression of the functional 64‐kDa γc. There was a similar loss of IL‐13Rα1 mRNA on monocyte differentiation. In turn, there was a significant reduction in the ability of IL‐4 and IL‐13 to activate STAT6. These findings suggest that different functional responses to IL‐4 and IL‐13 by human monocytes and macrophages may result from reduced expression of γc and IL‐13Rα1. J. Leukoc. Biol. 66: 575–578; 1999.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.66.4.575