Jasmonic acid influences mycorrhizal colonization in tomato plants by modifying the expression of genes involved in carbohydrate partitioning

The role of jasmonic acid (JA) on mycorrhizal colonization by Glomus fasciculatum in tomato plants was examined using mutant plants overexpressing prosystemin (PS) or affected in the synthesis of JA (suppressor of prosystemin-mediated responses 2, spr2). The degree of mycorrhizal colonization was de...

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Bibliographic Details
Published in:Physiologia plantarum 2008-06, Vol.133 (2), p.339-353
Main Authors: Tejeda-Sartorius, Miriam, Martínez de la Vega, Octavio, Délano-Frier, John Paul
Format: Article
Language:English
Subjects:
Acetates - pharmacology
beta-Fructofuranosidase - genetics
beta-Fructofuranosidase - metabolism
Biological and medical sciences
Carbohydrate Metabolism - genetics
Cell Wall - drug effects
Cell Wall - enzymology
Cyclopentanes - pharmacology
Fatty Acids - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant - drug effects
Genes, Plant
Glomus fasciculatum
Glucosyltransferases - genetics
Glucosyltransferases - metabolism
Lycopersicon esculentum
Lycopersicon esculentum - drug effects
Lycopersicon esculentum - genetics
Lycopersicon esculentum - microbiology
Metabolism
Mycorrhizae - drug effects
Oxylipins - pharmacology
Photosynthesis, respiration. Anabolism, catabolism
Plant physiology and development
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - drug effects
Plant Roots - genetics
Plant Roots - microbiology
Starch - metabolism
Sucrose - metabolism
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Summary:The role of jasmonic acid (JA) on mycorrhizal colonization by Glomus fasciculatum in tomato plants was examined using mutant plants overexpressing prosystemin (PS) or affected in the synthesis of JA (suppressor of prosystemin-mediated responses 2, spr2). The degree of mycorrhizal colonization was determined by measuring frequency (F%) and intensity (M%) of colonization and arbuscule abundance (A%). Gene expression and biochemical analyses were also performed in roots to detect changes in carbon (C) partitioning. Colonization was similar in mycorrhizal PS and wild-type roots, except for a higher A% in the former. Conversely, colonization was severely reduced in roots of spr2 mutants. No association was found between levels of expression of genes coding for systemic wound responsive proteins (or SWRPs) and other defense-related proteins in roots and mycorrhization levels in these plants. On the other hand, the degree of mycorrhizal colonization correlated with changes in the transcriptional regulation of a number of genes involved in sucrose hydrolysis and transport, cell wall invertase activity and mycorrhizal-specific fatty acid content in roots. The results obtained suggest that one of the mechanisms by which JA might operate to modulate the mycorrhization process could be through its influence on the regulation of C partitioning in the plant. The significant colonization increase observed in mycorrhizal spr2 plants supplied with exogenous methyl jasmonate supports its role as a positive regulator of the symbiosis.
ISSN:0031-9317
1399-3054
DOI:10.1111/j.1399-3054.2008.01081.x