A rapid recombination assay of HIV-1 using murine CD52 as a novel biomarker

Biomarkers are commonly used for verification of infection in conjunction with the development of viral vectors or experiments involving virus infection. Leukocyte surface antigens (CDs) are a prime option for biomarkers since they can be easily visualized and analyzed by flow cytometry after indire...

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Bibliographic Details
Published in:Microbes and infection 2008-04, Vol.10 (4), p.396-404
Main Authors: Sakuragi, Jun-ichi, Sakuragi, Sayuri, Ohishi, Masahisa, Shioda, Tatsuo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antigens, CD - analysis
Antigens, Neoplasm - analysis
Biological and medical sciences
Biomarker
Biomarkers - analysis
CD52
CD52 Antigen
Cell Line
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Glycoproteins - analysis
HIV-1 - genetics
Human immunodeficiency virus 1
Humans
Microbiology
Miscellaneous
Molecular Sequence Data
Recombination
Recombination, Genetic
Retrovector
Virology
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Summary:Biomarkers are commonly used for verification of infection in conjunction with the development of viral vectors or experiments involving virus infection. Leukocyte surface antigens (CDs) are a prime option for biomarkers since they can be easily visualized and analyzed by flow cytometry after indirect fluorescent staining. For analyses of human cells, murine CD24 (Heat Stable Antigen: HSA) and CD90.2 (Thy-1.2) are currently being used. In the study reported here, we attempted to develop a rapid system for measuring retroviral genome recombination efficiency. For this purpose, we looked for an alternative CD molecule which could be used as a marker on a viral vector concurrently with other markers. We found that murine CD52 is suitable for this purpose because of its small gene size, low inhibitory effect on virus production, and measurable level of surface expression. With this novel biomarker, we succeeded in developing a rapid viral recombination measuring system using a flow cytometer.
ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2007.12.016