Diagnosis of trypanosomiasis in experimental mice and field-infected camels by detection of antibody to trypanosome tyrosine aminotransferase

Sera from animals with acute and chronic Trypansoma evansi infections were examined directly for trypanosome tyrosine aminotransferase activity and indirectly for their ability to inhibit tyrosine aminotransferase activity. It was shown that sera from acutely infected mice and camels with high paras...

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Bibliographic Details
Published in:The Journal of parasitology 1998-12, Vol.84 (6), p.1245-1249
Main Authors: El Sawalhy, A, Seed, J.R
Format: Article
Language:English
Subjects:
Animal protozoal diseases
Animals
Antibodies
Antibodies, Protozoan - blood
Biological and medical sciences
Blood
Camels
Camelus
Camelus - parasitology
dromedaries
enzyme activity
Enzymes
experimental infections
Female
infection
Infections
Infectious diseases
Liver
Liver - enzymology
Male
Medical sciences
Mice
Parasitemia
Parasitemia - diagnosis
Parasitemia - enzymology
Parasitemia - veterinary
Parasitic diseases
Parasitology
Protozoal diseases
serodiagnosis
Therapeutics-Diagnostics
transaminases
Trypanosoma - enzymology
Trypanosoma - immunology
Trypanosoma evansi
Trypanosome
Trypanosomiasis, African - diagnosis
Trypanosomiasis, African - enzymology
Trypanosomiasis, African - veterinary
tyrosine
Tyrosine Transaminase - blood
Tyrosine Transaminase - immunology
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Summary:Sera from animals with acute and chronic Trypansoma evansi infections were examined directly for trypanosome tyrosine aminotransferase activity and indirectly for their ability to inhibit tyrosine aminotransferase activity. It was shown that sera from acutely infected mice and camels with high parasitemias contained significant levels of trypanosome tyrosine amino-transferase activity. In contrast, the sera from chronically infected mice and camels did not contain significant tyrosine amino-transferase activity, but they were able to neutralize the enzyme activity in trypanosome homogenates. The sera from camels with other pathological conditions did not neutralize this enzyme activity. It is suggested that the inhibitory factor in the chronic sera is antibody. The potential use of the direct enzyme assay and the indirect neutralization assay as diagnostic tools are discussed. Finally, the use of these assays to distinguish between early (acute) and late (chronic) infections are also suggested.
ISSN:0022-3395
1937-2345
DOI:10.2307/3284681