Ferulic acid provides neuroprotection against oxidative stress-related apoptosis after cerebral ischemia/reperfusion injury by inhibiting ICAM-1 mRNA expression in rats

Abstract Our previous studies have shown that ferulic acid (4-hydroxy-3-methoxycinnamic acid, FA) inhibits intercellular adhesion molecule-1 (ICAM-1) expression in the ischemic striatum after 2 h of reperfusion in a transient middle cerebral artery occlusion model in rats. The purpose of this study...

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Published in:Brain research 2008-05, Vol.1209, p.136-150
Main Authors: Cheng, Chin-Yi, Su, Shan-Yu, Tang, Nou-Ying, Ho, Tin-Yun, Chiang, Su-Yin, Hsieh, Ching-Liang
Format: Article
Language:English
Subjects:
4-Hydroxy-2-nonenal
8-Hydroxy-2′-deoxyguanosine
Aldehydes - metabolism
Animals
Apoptosis
Apoptosis - drug effects
Apoptosis - physiology
Biological and medical sciences
Brain Ischemia - drug therapy
Brain Ischemia - metabolism
Brain Ischemia - physiopathology
Caspase 3 - metabolism
Coumaric Acids - pharmacology
Coumaric Acids - therapeutic use
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - metabolism
Disease Models, Animal
Encephalitis - drug therapy
Encephalitis - metabolism
Encephalitis - physiopathology
Ferulic acid
Free Radical Scavengers - pharmacology
Free Radical Scavengers - therapeutic use
Gene Expression - drug effects
In Situ Nick-End Labeling
Infarction, Middle Cerebral Artery - drug therapy
Infarction, Middle Cerebral Artery - metabolism
Infarction, Middle Cerebral Artery - physiopathology
Intercellular Adhesion Molecule-1 - biosynthesis
Intercellular Adhesion Molecule-1 - genetics
Intercellular adhesion molecule-1 mRNA
Macrophage-1 Antigen - genetics
Macrophage-1 antigen mRNA
Male
Medical sciences
Nerve Tissue Proteins - metabolism
Neurology
Neuroprotective Agents - pharmacology
Neuroprotective Agents - therapeutic use
Nuclear Proteins - metabolism
Oxidative Stress - drug effects
Oxidative Stress - physiology
Rats
Rats, Sprague-Dawley
Reperfusion Injury - drug therapy
Reperfusion Injury - metabolism
Reperfusion Injury - physiopathology
RNA, Messenger - drug effects
RNA, Messenger - metabolism
Vascular diseases and vascular malformations of the nervous system
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Summary:Abstract Our previous studies have shown that ferulic acid (4-hydroxy-3-methoxycinnamic acid, FA) inhibits intercellular adhesion molecule-1 (ICAM-1) expression in the ischemic striatum after 2 h of reperfusion in a transient middle cerebral artery occlusion model in rats. The purpose of this study is to further investigate the neuroprotective effects of FA during reperfusion after cerebral ischemia. Rats were subjected to 90 min of ischemia; they were then sacrificed after 2, 10, 24 and 36 h of reperfusion. ICAM-1 and macrophage-1 antigen (Mac-1) mRNA were detected using semi-quantitative RT-PCR at 2 h of reperfusion. Mac-1, 4-hydroxy-2-nonenal (4-HNE), 8-hydroxy-2′-deoxyguanosine (8-OHdG), active caspase 3, neuronal nuclei (NeuN) and TUNEL positive cells were measured at 2, 10, 24 and 36 h of reperfusion. FA (100 mg/kg, i.v.) administered immediately after MCAo inhibited ICAM-1 and Mac-1 mRNA expression in the striatum at 2 h of reperfusion, and reduced the number of Mac-1, 4-HNE and 8-OHdG positive cells in the ischemic rim and core at 10, 24 and 36 h of reperfusion. FA decreased TUNEL positive cells in the penumbra at 10 h, and in the ischemic boundary and core at 24 and 36 h of reperfusion. FA curtailed active caspase 3 expression in the penumbra at 10 h and restored NeuN-labeled neurons in the penumbra and ischemic core at 36 h of reperfusion. FA decreased the level of ICAM-1 mRNA and the number of microglia/macrophages, and subsequently down-regulated inflammation-induced oxidative stress and oxidative stress-related apoptosis, suggesting that FA provides neuroprotection against oxidative stress-related apoptosis by inhibiting ICAM-1 mRNA expression after cerebral ischemia/reperfusion injury in rats.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2008.02.090