Glutathione-S-transferase M1 and T1 and cytochrome P1A1 genetic polymorphisms and susceptibility to cervical intraepithelial neoplasia in Greek women

The aim of the study was to determine the importance of genetic polymorphisms of glutathione-S-transferase T1 and M1 and cytochrome P1A1 genes in the development of cervical intraepithelial neoplasia in Greek women. This was a prospective, case-control study conducted by the Cervical Pathology and C...

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Bibliographic Details
Published in:European journal of cancer prevention 2007-12, Vol.16 (6), p.498-504
Main Authors: Agorastosa, Theodoros, Papadopoulos, Nikolaos, Lambropoulos, Alexandras F., Chrisafi, Sofia, Mikos, Themistoklis, Goulis, Dimitrios G., Constantinidis, Theodoros C., Kotsis, Alexandras, Bontis, John N.
Format: Article
Language:English
Subjects:
Adult
Case-Control Studies
Cervical Intraepithelial Neoplasia - etiology
Cervical Intraepithelial Neoplasia - genetics
Cervical Intraepithelial Neoplasia - pathology
Cytochrome P-450 CYP1A1 - genetics
Epidemiology
Female
Gene Frequency
Genetic Predisposition to Disease
Genotype
Glutathione Transferase - genetics
Greece
Humans
Polymorphism, Single Nucleotide
Prospective Studies
Smoking - adverse effects
Uterine Cervical Neoplasms - etiology
Uterine Cervical Neoplasms - genetics
Uterine Cervical Neoplasms - pathology
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Summary:The aim of the study was to determine the importance of genetic polymorphisms of glutathione-S-transferase T1 and M1 and cytochrome P1A1 genes in the development of cervical intraepithelial neoplasia in Greek women. This was a prospective, case-control study conducted by the Cervical Pathology and Colposcopy Unit of a University Ob/Gyn Department from 1999 to 2003. Cervical smears from 114 controls without any cytological and/or colposcopical evidence of cervical pathology and from 166 women with history of cervical intraepithelial neoplasia (56 CIN I, 54 CIN II and 56 CIN III) were examined with polymerase chain reaction for the abovementioned genetic polymorphisms, taking also in mind their smoking attitudes. Statistical analysis was performed to detect any association between the null genotype of GSTM1 and GSTT1 genes and the CYP1A1 ml polymorphism and the severity of cervical intraepithelial neoplasia. The distributions of the GSTT1 and GSTM1 wild-type genotypes were 57.48 and 39.75%, respectively. No woman with homozygous GSTT1 and GSTM1 null/null genotype was identified. CYP1A1 ml polymorphism frequency was 24.49%. No woman with homozygous CYP1A1 m1/m1 genotype was detected as well. No significant difference in the frequencies of the GSTM1 and GSTT1 null alleles, and the CYP1A1 m1 polymorphism, was found between cases and controls. After application of Mantel-Haenszel X² procedure, there was no linear severity of the lesion and the frequency of these polymorphisms. According to our results, glutathione-S-transferase T1 and M1 and cytochrome P1A1 genetic polymporphisms do not appear to be a risk factor for cervical disease irrespective of smoking habits.
ISSN:0959-8278
1473-5709
DOI:10.1097/01.cej.0000243859.99265.92