Nutritional and hormonal regulation of uncoupling protein gene expression in rat adipocytes

Nutritional and hormonal regulation of the expression of uncoupling protein (UCP)-1, -2, and -3 mRNA and protein was investigated in primary cultured adipocytes of rats. The UCP-1, -2, -3 mRNA and protein induction in the adipocytes reached maximal levels at 4 h in the presence of glucose with or wi...

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Bibliographic Details
Published in:Journal of Nutritional Science and Vitaminology 2007, Vol.53(5), pp.426-431
Main Authors: Fukuda, H.(Tezukayama Gakuin Coll., Sakai, Osaka (Japan)), Hirakawa, T, Iritani, N
Format: Article
Language:English
Subjects:
adipocytes
Adipocytes, Brown - drug effects
Adipocytes, Brown - metabolism
ADIPOSE TISSUE
Adrenergic Agonists - pharmacology
Analysis of Variance
Animals
ARN MENSAJERO
ARN MESSAGER
Biological and medical sciences
Blotting, Northern
Blotting, Western
CELL CULTURE
Cell Culture Techniques
CULTIVO DE CELULAS
CULTURE DE CELLULE
Dose-Response Relationship, Drug
Epinephrine - genetics
Epinephrine - pharmacology
EXPRESION GENICA
EXPRESSION DES GENES
Fatty Acids, Unsaturated - genetics
Fatty Acids, Unsaturated - pharmacology
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Gene Expression Regulation - drug effects
Gene Expression Regulation - genetics
Glucose - genetics
Glucose - pharmacology
HORMONAS
HORMONE
HORMONES
Hormones - genetics
Hormones - pharmacology
Hypoglycemic Agents - pharmacology
INSULIN
Insulin - genetics
Insulin - pharmacology
INSULINA
INSULINE
Ion Channels - drug effects
Ion Channels - genetics
MESSENGER RNA
MITOCHONDRIA
Mitochondrial Proteins - drug effects
Mitochondrial Proteins - genetics
MITOCHONDRIE
MITOCONDRIA
nutrient
NUTRIENTES
NUTRIENTS
PROTEINAS
PROTEINE
PROTEINS
rat
Rats
Rats, Wistar
RNA, Messenger - genetics
RNA, Messenger - metabolism
SUBSTANCE NUTRITIVE
TEJIDO ADIPOSO
Time Factors
TISSU ADIPEUX
Triiodothyronine - genetics
Triiodothyronine - pharmacology
uncoupling protein
Uncoupling Protein 1
Uncoupling Protein 2
Uncoupling Protein 3
Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue
VITAMINAS
VITAMINE
VITAMINS
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Summary:Nutritional and hormonal regulation of the expression of uncoupling protein (UCP)-1, -2, and -3 mRNA and protein was investigated in primary cultured adipocytes of rats. The UCP-1, -2, -3 mRNA and protein induction in the adipocytes reached maximal levels at 4 h in the presence of glucose with or without insulin. Moreover, the UCP induction was accelerated by triiodothyronine (T3) or epinephrine, and reached a maximum at 2 h. It appeared that the induction of UCP mRNA and protein was rapid. UCP-1 mRNA expression was stimulated by the presence of T3 or epinephrine in the culture medium. UCP-2 mRNA expression was more markedly increased by glucose, unsaturated fatty acids, insulin and T3 than UCP-1 or -3 mRNA expression. UCP-3 expression was more markedly increased by epinephrine than by T3. The protein expression of the UCPs was induced by glucose and the hormones nearly parallel to the UCP mRNA expression. Thus, UCP-2 expression appears to be stimulated by energy sources such as glucose and fat, and by regulators of thermogenesis and basal metabolic rate such as T3 and insulin, in contrast to UCP-1 and -3 expression.
ISSN:0301-4800
1881-7742
DOI:10.3177/jnsv.53.426