Human osteoblast-like cell adhesion on titanium substrates covalently functionalized with synthetic peptides

Abstract Biomaterials to be used for the production of endosseous devices in dental, orthopedic and maxillo-facial applications, might be designed to support, guide and enhance osteoblast adhesion. Cell recruitment onto biomaterial surface is a fundamental step within the complex process responsible...

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Bibliographic Details
Published in:Bone (New York, N.Y.) N.Y.), 2007-03, Vol.40 (3), p.693-699
Main Authors: Bagno, Andrea, Piovan, Alessandro, Dettin, Monica, Chiarion, Alessia, Brun, Paola, Gambaretto, Roberta, Fontana, Giovanni, Di Bello, Carlo, Palù, Giorgio, Castagliuolo, Ignazio
Format: Article
Language:English
Subjects:
Adhesion strength
Aged
Amino Acid Sequence
Biological and medical sciences
Cell adhesion
Cell Adhesion - physiology
Cell Adhesion Molecules - genetics
Coated Materials, Biocompatible - chemistry
Endosseous devices
Female
Fundamental and applied biological sciences. Psychology
Humans
Integrins - genetics
Male
Materials Testing
Middle Aged
Molecular Sequence Data
Orthopedics
Osteoblast-like cells
Osteoblasts - cytology
Proteoglycans - genetics
Surface Properties
Titanium
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:Abstract Biomaterials to be used for the production of endosseous devices in dental, orthopedic and maxillo-facial applications, might be designed to support, guide and enhance osteoblast adhesion. Cell recruitment onto biomaterial surface is a fundamental step within the complex process responsible for implant osseointegration; this process involves several proteins from the extra cellular matrix (ECM), cytoskeleton and cell membrane. A new strategy to improve endosseous implant integration is based on preparing biomimetic surfaces able to present adhesive factors to cells. Osteoblast adhesion takes place by at least two different mechanisms: the most investigated one implies the interaction with RGD sequences via cell-membrane integrin receptors; a further mechanism concerns the interaction between cell-membrane heparan sulfate proteoglycans and heparin-binding sites of ECM proteins. In the present study two different biomimetic surfaces were obtained by covalently grafting two adhesive peptides on oxidized titanium substrates after silanization: an RGD-containing peptide and a peptide mapped on human vitronectin. The two sequences are known to act via different adhesive mechanisms. The amount of human osteoblasts adhered onto peptide-enriched or not enriched titanium oxidized surfaces and the strength of cell binding were estimated, thus comparing the capacity of the bioactive substrates in promoting cell adhesion.
ISSN:8756-3282
1873-2763
DOI:10.1016/j.bone.2006.10.007