Lack of BK virus DNA sequences in most transitional‐cell carcinomas of the bladder

BK virus (BKV), a common human polyomavirus infection latent in the kidneys, can reactivate with immunosuppression to cause renal disease. Some have suggested that BKV may contribute to the development of bladder cancer, and BKV sequences have been reported from bladder tumors. To further examine th...

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Bibliographic Details
Published in:International journal of cancer 2007-03, Vol.120 (6), p.1248-1251
Main Authors: Rollison, Dana E., Sexton, Wade J., Rodriguez, Alejandro R., Kang, Loveleen C., Daniel, Richard, Shah, Keerti V.
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Antigens, Viral, Tumor - analysis
Antigens, Viral, Tumor - genetics
Biological and medical sciences
BK virus
BK Virus - genetics
BK Virus - immunology
BK Virus - isolation & purification
bladder cancer
Carcinoma, Transitional Cell - chemistry
Carcinoma, Transitional Cell - virology
DNA, Viral - analysis
Female
Humans
Male
Medical sciences
Middle Aged
Nephrology. Urinary tract diseases
PCR
Polymerase Chain Reaction
Polyomavirus
Polyomavirus Infections - complications
Tumor Virus Infections - complications
Tumors
Tumors of the urinary system
Urinary Bladder Neoplasms - chemistry
Urinary Bladder Neoplasms - virology
Urinary tract. Prostate gland
urothelial cancer
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Summary:BK virus (BKV), a common human polyomavirus infection latent in the kidneys, can reactivate with immunosuppression to cause renal disease. Some have suggested that BKV may contribute to the development of bladder cancer, and BKV sequences have been reported from bladder tumors. To further examine the role of BKV in human bladder cancer, a series of bladder tumors was investigated for BKV genomic sequences. Fresh‐frozen specimens from 76 transitional cell carcinoma tissues and 46 paired adjacent normal urothelial tissues archived at the H. Lee Moffitt Cancer Center were studied. All tissues were histopathologically reviewed. DNA extracted from the tissues was tested by quantitative real‐time polymerase chain reaction (QPCR) assays to detect BKV DNA sequences in the VP1 coding region. Amplification of ERV‐3 was conducted separately to quantify cell copy number. Conventional PCR targeting the BKV T‐antigen (T‐Ag) coding region and immunohistochemistry for BKV T‐Ag were also conducted on all tissues that tested positive for BKV by QPCR. Seventy‐three bladder tumors yielded ≥3,000 copies of ERV‐3, 4 (5.5%) of which tested positive for BKV with average copy numbers of 7.9, 15.8, 0.4 and 0.3 per 1,000 cells. Paired normal tissue was available for 2 of these BKV‐positive tumors, 1 of which was BKV‐positive (14.6 copies/1,000 cells). No other normal tissues were BKV‐positive by QPCR. The 6 BKV‐positive tissues by QPCR were also positive by conventional PCR, but all stained negative for BKV T‐Ag by immunohistochemistry. BKV is unlikely to be involved in the etiology of most bladder tumors. © 2006 Wiley‐Liss, Inc.
ISSN:0020-7136
1097-0215
DOI:10.1002/ijc.22494