Trichomonas vaginalis detection using real-time TaqMan PCR

1978 women and 93 men, all suspected of having a Trichomonas vaginalis infection, were tested for the presence of T. vaginalis by real-time PCR using the T. vaginalis-specific 2-kb repeated sequence, and by direct microscopy and culture. 40 samples were positive by T. vaginalis real-time PCR and 27...

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Bibliographic Details
Published in:Journal of microbiological methods 2007-02, Vol.68 (2), p.243-247
Main Authors: Schirm, Jurjen, Bos, Petra A.J., Roozeboom-Roelfsema, Irene K., Luijt, Dirk S., Möller, Lieke V.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
DNA, Protozoan - chemistry
DNA, Protozoan - genetics
Female
Human protozoal diseases
Humans
Infectious diseases
Male
Medical sciences
Netherlands
Parasitic diseases
Parasitic diseases due to ciliates and flagellates
Polymerase Chain Reaction - methods
Predictive Value of Tests
Protozoal diseases
Real-time PCR
Repetitive Sequences, Nucleic Acid - genetics
Sensitivity and Specificity
Trichomonas vaginalis
Trichomonas vaginalis - genetics
Trichomonas vaginalis - isolation & purification
Trichomonas Vaginitis - parasitology
Tubulin - chemistry
Tubulin - genetics
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Summary:1978 women and 93 men, all suspected of having a Trichomonas vaginalis infection, were tested for the presence of T. vaginalis by real-time PCR using the T. vaginalis-specific 2-kb repeated sequence, and by direct microscopy and culture. 40 samples were positive by T. vaginalis real-time PCR and 27 were positive by wet mount microscopy, either direct or after culture. All samples positive by direct microscopy of culture were also positive by real-time PCR. Of the 13 samples which were real-time PCR positive but negative by direct microscopy and culture 11 were confirmed by another T. vaginalis real-time PCR based on the beta tubulin gene. Only 2 samples (0.1%) showed inhibition in the PCR. The prevalence of T. vaginalis infection in the female patients was 1.8%. The sensitivity, specificity, positive and negative predictive values of the real-time PCR were 100%, 99.9%, 95% and 100%, respectively. The same test characteristics for the combined conventional T. vaginalis detection methods (microscopy + culture) were 71%, 100%, 100% and 99%, respectively. Therefore, real-time PCR is the method of choice for the diagnosis of T. vaginalis infection.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2006.08.002