Peripheral Blood Stem Cell Tumor Cell Contamination and Survival of Neuroblastoma Patients

Purpose: Contribution of peripheral blood stem cell (PBSC) contaminating tumor cells to subsequent relapse and overall survival of neuroblastoma patients remains controversial. Experimental Design: Neuroblastoma cell contamination of 27 PBSC harvests from stage IV neuroblastoma patients was assessed...

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Bibliographic Details
Published in:Clinical cancer research 2006-10, Vol.12 (19), p.5680-5685
Main Authors: CORRIAS, Maria Valeria, HAUPT, Riccardo, CARLINI, Barbara, PARODI, Stefano, RIVABELLA, Lucia, GARAVENTA, Alberto, PISTOIA, Vito, DAIIORSO, Sandro
Format: Article
Language:English
Subjects:
Antineoplastic agents
Biological and medical sciences
Bone Marrow Purging - methods
Cell Separation - methods
Child, Preschool
Disease-Free Survival
Female
GD2 synthase
Hematopoietic Stem Cells - pathology
Humans
Male
Medical sciences
N-Acetylgalactosaminyltransferases - genetics
N-Acetylgalactosaminyltransferases - metabolism
Neoplastic Cells, Circulating - pathology
Neuroblastoma - enzymology
Neuroblastoma - mortality
Neuroblastoma - therapy
Neurology
PBSC contamination
Pharmacology. Drug treatments
Polymerase Chain Reaction - methods
quantitative RT-PCR
RNA, Neoplasm - genetics
RNA, Neoplasm - metabolism
Survival Rate
Transplantation, Autologous
tumor detection
Tumors of the nervous system. Phacomatoses
Tyrosine 3-Monooxygenase - genetics
Tyrosine 3-Monooxygenase - metabolism
tyrosine hydroxylase
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Summary:Purpose: Contribution of peripheral blood stem cell (PBSC) contaminating tumor cells to subsequent relapse and overall survival of neuroblastoma patients remains controversial. Experimental Design: Neuroblastoma cell contamination of 27 PBSC harvests from stage IV neuroblastoma patients was assessed by quantitative RT-PCR for both tyrosine hydroxylase (TH) and GD2 synthase (GD2-s). The effect of PBSC contamination on survival was then analyzed. Results: Seven PBSC tested negative for both markers; 19 were positive for GD2-s, 6 for TH, with 5 positive for both. Survival of the 20 patients with positive PBSC did not differ from that of the patients with negative PBSC (log-rank test, P = 0.134 and 0.218 for event-free survival and overall survival, respectively). By considering the TH and GD2-s results independently, a borderline ( P = 0.053) negative effect on event-free survival was observed in patients reinfused with GD2-s-positive PBSC. When the status at transplant was taken into account, only the event-free survival of the patients rescued when in complete remission with GD2-s-negative PBSC was better, although not significantly, than that of patients infused with GD2-s-positive PBSC. Conclusions: Our results obtained in a small cohort of homogeneously treated stage IV patients suggest that patient survival is not affected by PBSC contamination with the exception of a borderline negative effect on event-free survival in patients rescued when in complete remission.
ISSN:1078-0432
1557-3265
DOI:10.1158/1078-0432.CCR-06-0740